論文 - 詳細
| RRC ID | 22006 |
|---|---|
| 著者 | McClure KD, Heberlein U. |
| タイトル | A small group of neurosecretory cells expressing the transcriptional regulator apontic and the neuropeptide corazonin mediate ethanol sedation in Drosophila. |
| ジャーナル | J Neurosci |
| Abstract |
In the fruit fly Drosophila melanogaster, as in mammals, acute exposure to a high dose of ethanol leads to stereotypical behavioral changes beginning with increased activity, followed by incoordination, loss of postural control, and eventually, sedation. The mechanism(s) by which ethanol impacts the CNS leading to ethanol-induced sedation and the genes required for normal sedation sensitivity remain largely unknown. Here we identify the gene apontic (apt), an Myb/SANT-containing transcription factor that is required in the nervous system for normal sensitivity to ethanol sedation. Using genetic and behavioral analyses, we show that apt mediates sensitivity to ethanol sedation by acting in a small set of neurons that express Corazonin (Crz), a neuropeptide likely involved in the physiological response to stress. The activity of Crz neurons regulates the behavioral response to ethanol, as silencing and activating these neurons affects sedation sensitivity in opposite ways. Furthermore, this effect is mediated by Crz, as flies with reduced crz expression show reduced sensitivity to ethanol sedation. Finally, we find that both apt and crz are rapidly upregulated by acute ethanol exposure. Thus, we have identified two genes and a small set of peptidergic neurons that regulate sensitivity to ethanol-induced sedation. We propose that Apt regulates the activity of Crz neurons and/or release of the neuropeptide during ethanol exposure. |
| 巻・号 | 33(9) |
| ページ | 4044-54 |
| 公開日 | 2013-2-27 |
| DOI | 10.1523/JNEUROSCI.3413-12.2013 |
| PII | 33/9/4044 |
| PMID | 23447613 |
| PMC | PMC3865498 |
| MeSH | Animals Animals, Genetically Modified Behavior, Animal / drug effects Brain / cytology DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Dose-Response Relationship, Drug Drosophila / genetics Drosophila Proteins / genetics Drosophila Proteins / metabolism* Ethanol / pharmacology* Gene Expression Regulation / drug effects* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Hypnotics and Sedatives / pharmacology* Metamorphosis, Biological / genetics Mutation / genetics Neurons / drug effects* Neurons / metabolism Neuropeptides / genetics Neuropeptides / metabolism* Neurosecretory Systems / cytology* RNA, Messenger / metabolism Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 5.674 |
| 引用数 | 19 |
| WOS 分野 | NEUROSCIENCES |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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