RRC ID 22031
著者 Minami R, Wakabayashi M, Sugimori S, Taniguchi K, Kokuryo A, Imano T, Adachi-Yamada T, Watanabe N, Nakagoshi H.
タイトル The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila.
ジャーナル PLoS One
Abstract The Drosophila male accessory gland has functions similar to those of the mammalian prostate gland and the seminal vesicle, and secretes accessory gland proteins into the seminal fluid. Each of the two lobes of the accessory gland is composed of two types of binucleate cell: about 1,000 main cells and 40 secondary cells. A well-known accessory gland protein, sex peptide, is secreted from the main cells and induces female postmating response to increase progeny production, whereas little is known about physiological significance of the secondary cells. The homeodomain transcriptional repressor Defective proventriculus (Dve) is strongly expressed in adult secondary cells, and its mutation resulted in loss of secondary cells, mononucleation of main cells, and reduced size of the accessory gland. dve mutant males had low fecundity despite the presence of sex peptide, and failed to induce the female postmating responses of increased egg laying and reduced sexual receptivity. RNAi-mediated dve knockdown males also had low fecundity with normally binucleate main cells. We provide the first evidence that secondary cells are crucial for male fecundity, and also that Dve activity is required for survival of the secondary cells. These findings provide new insights into a mechanism of fertility/fecundity.
巻・号 7(3)
ページ e32302
公開日 2012-3-12
DOI 10.1371/journal.pone.0032302
PII PONE-D-11-22990
解説 PMC3299662
PMID 22427829
PMC PMC3299662
MeSH Analysis of Variance Animals Drosophila / metabolism Drosophila / physiology* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Exocrine Glands / cytology Exocrine Glands / growth & development Exocrine Glands / metabolism* Female Fertility / physiology Gene Expression Profiling Genetic Vectors / genetics Genitalia, Male / cytology Genitalia, Male / growth & development Genitalia, Male / metabolism* Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Immunohistochemistry Intercellular Signaling Peptides and Proteins Male Peptides / metabolism RNA Interference Semen / metabolism Semen / physiology Sexual Behavior, Animal / physiology Transcription Factors / genetics
IF 2.74
引用数 20
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ protamineB::GFP (DGRC#109173 or DGRC#109175)