Reference - Detail
| RRC ID | 22034 |
|---|---|
| Author | Timofeev K, Joly W, Hadjieconomou D, Salecker I. |
| Title | Localized netrins act as positional cues to control layer-specific targeting of photoreceptor axons in Drosophila. |
| Journal | Neuron |
| Abstract |
A shared feature of many neural circuits is their organization into synaptic layers. However, the mechanisms that direct neurites to distinct layers remain poorly understood. We identified a central role for Netrins and their receptor Frazzled in mediating layer-specific axon targeting in the Drosophila visual system. Frazzled is expressed and cell autonomously required in R8 photoreceptors for directing their axons to the medulla-neuropil layer M3. Netrin-B is specifically localized in this layer owing to axonal release by lamina neurons L3 and capture by target neuron-associated Frazzled. Ligand expression in L3 is sufficient to rescue R8 axon-targeting defects of Netrin mutants. R8 axons target normally despite replacement of diffusible Netrin-B by membrane-tethered ligands. Finally, Netrin localization is instructive because expression in ectopic layers can retarget R8 axons. We propose that provision of localized chemoattractants by intermediate target neurons represents a highly precise strategy to direct axons to a positionally defined layer. |
| Volume | 75(1) |
| Pages | 80-93 |
| Published | 2012-7-12 |
| DOI | 10.1016/j.neuron.2012.04.037 |
| PII | S0896-6273(12)00442-4 |
| Description | PMC3398394 |
| PMID | 22794263 |
| PMC | PMC3398394 |
| MeSH | Animals Animals, Genetically Modified Axons / chemistry Axons / metabolism* Cues* Drosophila Proteins / chemistry Drosophila Proteins / metabolism* Drosophila melanogaster Nerve Growth Factors / biosynthesis Nerve Growth Factors / metabolism* Netrin Receptors Netrins Photoreceptor Cells, Invertebrate / chemistry Photoreceptor Cells, Invertebrate / metabolism* Receptors, Cell Surface / biosynthesis* Visual Pathways / chemistry Visual Pathways / metabolism |
| IF | 14.415 |
| Times Cited | 58 |
| WOS Category | NEUROSCIENCES |
| Resource | |
| Drosophila | |