RRC ID 22044
Author Rozhkov NV, Hammell M, Hannon GJ.
Title Multiple roles for Piwi in silencing Drosophila transposons.
Journal Genes Dev.
Abstract Silencing of transposons in the Drosophila ovary relies on three Piwi family proteins--Piwi, Aubergine (Aub), and Ago3--acting in concert with their small RNA guides, the Piwi-interacting RNAs (piRNAs). Aub and Ago3 are found in the germ cell cytoplasm, where they function in the ping-pong cycle to consume transposon mRNAs. The nuclear Piwi protein is required for transposon silencing in both germ and somatic follicle cells, yet the precise mechanisms by which Piwi acts remain largely unclear. We investigated the role of Piwi by combining cell type-specific knockdowns with measurements of steady-state transposon mRNA levels, nascent RNA synthesis, chromatin state, and small RNA abundance. In somatic cells, Piwi loss led to concerted effects on nascent transcripts and transposon mRNAs, indicating that Piwi acts through transcriptional gene silencing (TGS). In germ cells, Piwi loss showed disproportionate impacts on steady-state RNA levels, indicating that it also exerts an effect on post-transcriptional gene silencing (PTGS). Piwi knockdown affected levels of germ cell piRNAs presumably bound to Aub and Ago3, perhaps explaining its post-transcriptional impacts. Overall, our results indicate that Piwi plays multiple roles in the piRNA pathway, in part enforcing transposon repression through effects on local chromatin states and transcription but also participating in germ cell piRNA biogenesis.
Volume 27(4)
Pages 400-12
Published 2013-2-15
DOI 10.1101/gad.209767.112
PII gad.209767.112
Description PMC3589557
PMID 23392609
PMC PMC3589557
MeSH Animals Argonaute Proteins / genetics* Argonaute Proteins / metabolism DNA Transposable Elements / genetics* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism* Female Gene Knockdown Techniques Ovary / metabolism Peptide Initiation Factors / genetics Peptide Initiation Factors / metabolism RNA Interference* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism
IF 9.462
Times Cited 93
Drosophila DGRC#104055