RRC ID 2224
Author Ohtaka A, Okuzaki D, Saito TT, Nojima H.
Title Mcp4, a meiotic coiled-coil protein, plays a role in F-actin positioning during Schizosaccharomyces pombe meiosis.
Journal Eukaryot Cell
Abstract Some meiosis-specific proteins of Schizosaccharomyces pombe harbor coiled-coil motifs and play essential roles in meiotic progression. Here we describe Mcp4, a novel meiosis-specific protein whose expression is abruptly induced at the horsetail phase and which remains expressed until sporulation is finished. Fluorescence microscopic analysis revealed that Mcp4 alters its subcellular localization during meiosis in a manner that partially resembles the movement of F-actin during meiosis. Mcp4 and F-actin never colocalize; rather, they are located in a side-by-side manner. When forespore membrane formation begins at metaphase II, the Mcp4 signals assemble at the lagging face of the dividing nuclei. At this stage, they are sandwiched between F-actin and the nucleus. Mcp4, in turn, appears to sandwich F-actin with Meu14. In mcp4Delta cells at anaphase II, the F-actin, which is normally dumbbell-shaped, adopts an abnormal balloon shape. Spores of mcp4Delta cells were sensitive to NaCl, although their shape and viability were normal. Taken together, we conclude that Mcp4 plays a role in the accurate positioning of F-actin during S. pombe meiosis.
Volume 6(6)
Pages 971-83
Published 2007-6-1
DOI 10.1128/EC.00016-07
PII EC.00016-07
PMID 17435009
PMC PMC1951525
MeSH Actins / metabolism* Meiosis / physiology* Phenotype Protein Structure, Tertiary Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spores, Fungal / metabolism
IF 2.992
Times Cited 8
Yeast CD16-1 TP4-5A TP4-1D YN68 FY13596 FY7051(JZ670)