RRC ID 2245
Author Isono E, Nishihara K, Saeki Y, Yashiroda H, Kamata N, Ge L, Ueda T, Kikuchi Y, Tanaka K, Nakano A, Toh-e A.
Title The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome.
Journal Mol. Biol. Cell
Abstract The 26S proteasome consists of the 20S proteasome (core particle) and the 19S regulatory particle made of the base and lid substructures, and it is mainly localized in the nucleus in yeast. To examine how and where this huge enzyme complex is assembled, we performed biochemical and microscopic characterization of proteasomes produced in two lid mutants, rpn5-1 and rpn7-3, and a base mutant DeltaN rpn2, of the yeast Saccharomyces cerevisiae. We found that, although lid formation was abolished in rpn5-1 mutant cells at the restrictive temperature, an apparently intact base was produced and localized in the nucleus. In contrast, in DeltaN rpn2 cells, a free lid was formed and localized in the nucleus even at the restrictive temperature. These results indicate that the modules of the 26S proteasome, namely, the core particle, base, and lid, can be formed and imported into the nucleus independently of each other. Based on these observations, we propose a model for the assembly process of the yeast 26S proteasome.
Volume 18(2)
Pages 569-80
Published 2007-2
DOI 10.1091/mbc.e06-07-0635
PII E06-07-0635
PMID 17135287
PMC PMC1783769
MeSH Active Transport, Cell Nucleus Cell Nucleus / enzymology Hot Temperature Models, Molecular Proteasome Endopeptidase Complex / analysis Proteasome Endopeptidase Complex / chemistry Proteasome Endopeptidase Complex / genetics Proteasome Endopeptidase Complex / metabolism* Saccharomyces cerevisiae / enzymology* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / analysis Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism*
IF 3.905
Times Cited 65
Yeast YAT3507 (=BY24003)