RRC ID 2247
著者 Hirasaki M, Kaneko Y, Harashima S.
タイトル Protein phosphatase Siw14 controls intracellular localization of Gln3 in cooperation with Npr1 kinase in Saccharomyces cerevisiae.
ジャーナル Gene
Abstract Saccharomyces cerevisiae Deltasiw14 disruptant exhibits caffeine sensitivity. To understand the function of Siw14, double disruptants for SIW14 and each of 102 viable protein kinases (PKase) genes were constructed and examined for suppression of caffeine sensitivity based on the premise that the sensitivity was caused either by accumulation of an unknown phosphorylated Siw14 substrate(s) or by depletion of an unphosphorylated substrate(s) of Siw14 in the Deltasiw14 disruptant. Among 102 pkase disruptions, only one, Deltanpr1, suppressed the caffeine sensitivity of the Deltasiw14 disruptant. Because Gln3 (a phosphorylated transcriptional activator)-dependent transcription is induced by disruption of NPR1, we further examined the effect of disruption and overexpression of GLN3 on the caffeine sensitivity of the Deltasiw14 disruptant. Disruption of GLN3 was found to partially suppress the caffeine sensitivity of the Deltasiw14 disruptant, while overexpression of GLN3 in wild-type cells caused caffeine sensitivity, providing the first evidence that Siw14 functions in the Gln3 regulatory network. We also found that, unlike in a wild-type background, Gln3 accumulates in the nucleus whether cells are exposed or not to caffeine in the Deltasiw14 disruptant, and that this nuclear localization was abolished by disruption of NPR1. Interestingly, the level of Gln3 phosphorylation in both the Deltasiw14 and Deltanpr1 disruptants decreased relative to wild type, independent of exposure to caffeine. We conclude that Siw14 controls the intracellular localization of Gln3 in combination with Npr1, and one of the causes for the caffeine sensitivity of the Deltasiw14 disruptant was an accumulation of dephosphorylated Gln3 in the nucleus.
巻・号 409(1-2)
ページ 34-43
公開日 2008-1-2
DOI 10.1016/j.gene.2007.11.005
PII S0378-1119(07)00569-0
PMID 18166280
MeSH Caffeine / pharmacology* Cell Compartmentation Gene Expression Profiling Gene Expression Regulation, Fungal Phosphorylation Protein Kinases / physiology* Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / physiology* Repressor Proteins / metabolism* Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Saccharomyces cerevisiae Proteins / physiology* Transcription Factors / metabolism*
IF 2.415
引用数 7
酵母 BY23850(BY4742) BY5209(SH5209 FY833) BY5210(SH5210 FY834)