論文 - 詳細
| RRC ID | 22727 |
|---|---|
| 著者 | Iwaki A, Izawa S. |
| タイトル | Acidic stress induces the formation of P-bodies, but not stress granules, with mild attenuation of bulk translation in Saccharomyces cerevisiae. |
| ジャーナル | Biochem J |
| Abstract |
The stress response of eukaryotic cells often causes an attenuation of bulk translation activity and the accumulation of non-translating mRNAs into cytoplasmic mRNP (messenger ribonucleoprotein) granules termed cytoplasmic P-bodies (processing bodies) and SGs (stress granules). We examined effects of acidic stress on the formation of mRNP granules compared with other forms of stress such as glucose deprivation and a high Ca²⁺ level in Saccharomyces cerevisiae. Treatment with lactic acid clearly caused the formation of P-bodies, but not SGs, and also caused an attenuation of translation initiation, albeit to a lesser extent than glucose depletion. P-body formation was also induced by hydrochloric acid and sulfuric acid. However, lactic acid in SD (synthetic dextrose) medium with a pH greater than 3.0, propionic acid and acetic acid did not induce P-body formation. The results of the present study suggest that the assembly of yeast P-bodies can be induced by external conditions with a low pH and the threshold was around pH 2.5. The P-body formation upon acidic stress required Scd6 (suppressor of clathrin deficiency 6), a component of P-bodies, indicating that P-bodies induced by acidic stress have rules of assembly different from those induced by glucose deprivation or high Ca²⁺ levels. |
| 巻・号 | 446(2) |
| ページ | 225-33 |
| 公開日 | 2012-9-1 |
| DOI | 10.1042/BJ20120583 |
| PII | BJ20120583 |
| PMID | 22686455 |
| MeSH | Carboxylic Acids / pharmacology* Clathrin / metabolism Cytoplasmic Granules / drug effects* Cytoplasmic Granules / metabolism Cytoskeletal Proteins / biosynthesis Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism Food Preservatives / pharmacology* Fungicides, Industrial / pharmacology* Hydrogen-Ion Concentration Microbial Viability / drug effects Microscopy, Fluorescence Osmolar Concentration Polyribosomes / drug effects Polyribosomes / metabolism Protein Biosynthesis / drug effects RNA Cap-Binding Proteins / biosynthesis RNA Cap-Binding Proteins / genetics RNA Cap-Binding Proteins / metabolism RNA, Fungal / metabolism RNA, Messenger / metabolism Recombinant Proteins / biosynthesis Recombinant Proteins / metabolism Ribonucleoproteins / metabolism Saccharomyces cerevisiae / drug effects* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / biosynthesis* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Stress, Physiological |
| IF | 4.097 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 酵母 | NA |