RRC ID 22741
著者 Tatsuke T, Lee JM, Kusakabe T, Iiyama K, Sezutsu H, Uchino K.
タイトル Tightly controlled tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells.
ジャーナル Arch Insect Biochem Physiol
Abstract Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promoters for a Bombyx mori Tet-on system. In order to further improve this system, Giant, a transcriptional silencer from Drosophila melanogaster, is introduced to repress leaky transcription in the absence of doxycycline. Further, the promoter responsibility to the tetracycline-controlled transactivators is facilitated by introducing a synthetic minimal core promoter. With the tightly regulated second-generation silkworm Tet-on system, we obtain up to 300-fold induction of gene expression with the addition of doxycycline.
巻・号 82(4)
ページ 173-82
公開日 2013-4-1
DOI 10.1002/arch.21083
PMID 23371880
MeSH Animals Bombyx / cytology Bombyx / metabolism* Cells, Cultured Gene Expression Regulation Genes, Reporter Genetic Techniques* Nuclear Localization Signals Promoter Regions, Genetic RNA Interference Tetracycline Trans-Activators* Transcription, Genetic*
IF 1.536
引用数 3
WOS 分野 ENTOMOLOGY PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
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