論文 - 詳細
| RRC ID | 22741 |
|---|---|
| 著者 | Tatsuke T, Lee JM, Kusakabe T, Iiyama K, Sezutsu H, Uchino K. |
| タイトル | Tightly controlled tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells. |
| ジャーナル | Arch Insect Biochem Physiol |
| Abstract |
Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promoters for a Bombyx mori Tet-on system. In order to further improve this system, Giant, a transcriptional silencer from Drosophila melanogaster, is introduced to repress leaky transcription in the absence of doxycycline. Further, the promoter responsibility to the tetracycline-controlled transactivators is facilitated by introducing a synthetic minimal core promoter. With the tightly regulated second-generation silkworm Tet-on system, we obtain up to 300-fold induction of gene expression with the addition of doxycycline. |
| 巻・号 | 82(4) |
| ページ | 173-82 |
| 公開日 | 2013-4-1 |
| DOI | 10.1002/arch.21083 |
| PMID | 23371880 |
| MeSH | Animals Bombyx / cytology Bombyx / metabolism* Cells, Cultured Gene Expression Regulation Genes, Reporter Genetic Techniques* Nuclear Localization Signals Promoter Regions, Genetic RNA Interference Tetracycline Trans-Activators* Transcription, Genetic* |
| IF | 1.536 |
| 引用数 | 3 |
| WOS 分野 | ENTOMOLOGY PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
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