RRC ID |
22754
|
著者 |
Ito K, Kidokoro K, Katsuma S, Shimada T, Yamamoto K, Mita K, Kadono-Okuda K.
|
タイトル |
Positional cloning of a gene responsible for the cts mutation of the silkworm, Bombyx mori.
|
ジャーナル |
Genome
|
Abstract |
The larval head cuticle and anal plates of the silkworm mutant cheek and tail spot (cts) have chocolate-colored spots, unlike the entirely white appearance of the wild-type (WT) strain. We report the identification and characterization of the gene responsible for the cts mutation. Positional cloning revealed a cts candidate on chromosome 16, designated BmMFS, based on the high similarity of the deduced amino acid sequence between the candidate gene from the WT strain and the major facilitator superfamily (MFS) protein. BmMFS likely encodes a membrane protein with 11 putative transmembrane domains, while the putative structure deduced from the cts-type allele possesses only 10-pass transmembrane domains owing to a deletion in its coding region. Quantitative RT-PCR analysis showed that BmMFS mRNA was strongly expressed in the integument of the head and tail, where the cts phenotype is observed; expression markedly increased at the molting and newly ecdysed stages. These results indicate that the novel BmMFS gene is cts and the membrane structure of its protein accounts for the cts phenotype. These expression profiles and the cts phenotype are quite similar to those of melanin-related genes, such as Bmyellow-e and Bm-iAANAT, suggesting that BmMFS is involved in the melanin synthesis pathway.
|
巻・号 |
55(7)
|
ページ |
493-504
|
公開日 |
2012-7-1
|
DOI |
10.1139/g2012-033
|
解説 |
cts(頬尾斑)の原因遺伝子はBmMFSである。BmMFSはMFS familyに属するタンパク質(BmMSF)ををコードする。i01、 w16、およびw41系統のcts個体では、BmMFSのエクソン7を含む約6kbの欠失が存在する。
|
PMID |
22703239
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Bombyx / genetics*
Bombyx / metabolism
Cloning, Organism
Genes, Insect
Insect Proteins / genetics*
Insect Proteins / metabolism
Molecular Sequence Data
Mutation*
Phenotype
RNA, Messenger / metabolism
Sequence Alignment
Sequence Analysis, DNA
|
IF |
2.037
|
引用数 |
10
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
リソース情報 |
カイコ |
i01
i02
w16
w41
t32 |