RRC ID |
22757
|
著者 |
Kawaoka S, Mitsutake H, Kiuchi T, Kobayashi M, Yoshikawa M, Suzuki Y, Sugano S, Shimada T, Kobayashi J, Tomari Y, Katsuma S.
|
タイトル |
A role for transcription from a piRNA cluster in de novo piRNA production.
|
ジャーナル |
RNA
|
Abstract |
PIWI-interacting RNAs (piRNAs) are at the heart of the nucleic acid-based adaptive immune system against transposons in animal gonads. To date, how the piRNA pathway senses an element as a substrate and how de novo piRNA production is initiated remain elusive. Here, by utilizing a GFP transgene, we screened and obtained clonal silkworm BmN4 cell lines producing massively amplified GFP-derived piRNAs capable of silencing GFP in trans. In multiple independent cell lines where GFP expression was silenced by the piRNA pathway, we detected a common transcript from an endogenous piRNA cluster, in which a part of the cluster is uniquely fused with an antisense GFP sequence. Bioinformatic analyses suggest that the fusion transcript is a source of GFP primary piRNAs. Our data implicate a role for transcription from a piRNA cluster in initiating de novo piRNA production against a new insertion.
|
巻・号 |
18(2)
|
ページ |
265-73
|
公開日 |
2012-2-1
|
DOI |
10.1261/rna.029777.111
|
PII |
rna.029777.111
|
PMID |
22194309
|
PMC |
PMC3264913
|
MeSH |
Animals
Bombyx / genetics
Bombyx / metabolism
Cell Line
Female
Gene Silencing
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Ovary / metabolism
RNA Splicing / genetics
RNA, Small Interfering / biosynthesis*
RNA, Small Interfering / genetics*
RNA, Small Interfering / metabolism
Transgenes
|
IF |
4.32
|
引用数 |
29
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
カイコ |
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