論文 - 詳細
| RRC ID | 22757 |
|---|---|
| 著者 | Kawaoka S, Mitsutake H, Kiuchi T, Kobayashi M, Yoshikawa M, Suzuki Y, Sugano S, Shimada T, Kobayashi J, Tomari Y, Katsuma S. |
| タイトル | A role for transcription from a piRNA cluster in de novo piRNA production. |
| ジャーナル | RNA |
| Abstract |
PIWI-interacting RNAs (piRNAs) are at the heart of the nucleic acid-based adaptive immune system against transposons in animal gonads. To date, how the piRNA pathway senses an element as a substrate and how de novo piRNA production is initiated remain elusive. Here, by utilizing a GFP transgene, we screened and obtained clonal silkworm BmN4 cell lines producing massively amplified GFP-derived piRNAs capable of silencing GFP in trans. In multiple independent cell lines where GFP expression was silenced by the piRNA pathway, we detected a common transcript from an endogenous piRNA cluster, in which a part of the cluster is uniquely fused with an antisense GFP sequence. Bioinformatic analyses suggest that the fusion transcript is a source of GFP primary piRNAs. Our data implicate a role for transcription from a piRNA cluster in initiating de novo piRNA production against a new insertion. |
| 巻・号 | 18(2) |
| ページ | 265-73 |
| 公開日 | 2012-2-1 |
| DOI | 10.1261/rna.029777.111 |
| PII | rna.029777.111 |
| PMID | 22194309 |
| PMC | PMC3264913 |
| MeSH | Animals Bombyx / genetics Bombyx / metabolism Cell Line Female Gene Silencing Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Ovary / metabolism RNA Splicing / genetics RNA, Small Interfering / biosynthesis* RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism Transgenes |
| IF | 4.32 |
| 引用数 | 29 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | |