RRC ID 22757
Author Kawaoka S, Mitsutake H, Kiuchi T, Kobayashi M, Yoshikawa M, Suzuki Y, Sugano S, Shimada T, Kobayashi J, Tomari Y, Katsuma S.
Title A role for transcription from a piRNA cluster in de novo piRNA production.
Journal RNA
Abstract PIWI-interacting RNAs (piRNAs) are at the heart of the nucleic acid-based adaptive immune system against transposons in animal gonads. To date, how the piRNA pathway senses an element as a substrate and how de novo piRNA production is initiated remain elusive. Here, by utilizing a GFP transgene, we screened and obtained clonal silkworm BmN4 cell lines producing massively amplified GFP-derived piRNAs capable of silencing GFP in trans. In multiple independent cell lines where GFP expression was silenced by the piRNA pathway, we detected a common transcript from an endogenous piRNA cluster, in which a part of the cluster is uniquely fused with an antisense GFP sequence. Bioinformatic analyses suggest that the fusion transcript is a source of GFP primary piRNAs. Our data implicate a role for transcription from a piRNA cluster in initiating de novo piRNA production against a new insertion.
Volume 18(2)
Pages 265-73
Published 2012-2
DOI 10.1261/rna.029777.111
PII rna.029777.111
PMID 22194309
PMC PMC3264913
MeSH Animals Bombyx / genetics Bombyx / metabolism Cell Line Female Gene Silencing Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Ovary / metabolism RNA Splicing / genetics RNA, Small Interfering / biosynthesis* RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism Transgenes
IF 4.49
Times Cited 20