論文 - 詳細
| RRC ID | 22760 |
|---|---|
| 著者 | Ando T, Fujiwara H. |
| タイトル | Electroporation-mediated somatic transgenesis for rapid functional analysis in insects. |
| ジャーナル | Development |
| Abstract |
Transgenesis is a powerful technique for determining gene function; however, it is time-consuming. It is virtually impossible to carry out in non-model insects in which egg manipulation and screening are difficult. We have established a rapid genetic functional analysis system for non-model insects using a low-cost electroporator (costing under US$200) designed for somatic transformation with the piggyBac transposon. Using this system, we successfully generated somatic transgenic cell clones in various target tissues (e.g. olfactory neurons, wing epidermis, larval epidermis, muscle, fat body and trachea) of the silkworm Bombyx mori during development. We also induced stable and transient RNA interference (RNAi) using short hairpin RNA (shRNA)-mediating DNA vectors and direct transfer of small interfering RNAs (siRNAs), respectively. We found that these electroporation-mediated approaches could also be applied to the swallowtail butterfly Papilio xuthus and the red flour beetle Tribolium castaneum. Thus, this method could be a powerful genetic tool for elucidating various developmental phenomena in non-model insects. |
| 巻・号 | 140(2) |
| ページ | 454-8 |
| 公開日 | 2013-1-15 |
| DOI | 10.1242/dev.085241 |
| PII | 140/2/454 |
| PMID | 23250219 |
| MeSH | Animals Bombyx DNA Transposable Elements Electroporation / methods* Gene Expression Regulation, Developmental Gene Transfer Techniques Genetic Vectors Insecta Microscopy, Fluorescence / methods Models, Biological Models, Genetic RNA Interference RNA, Small Interfering / metabolism Somites / metabolism* Transgenes* Tribolium / metabolism |
| IF | 5.611 |
| 引用数 | 38 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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