RRC ID 2277
Author Kamei K, Ishikawa TO, Herschman HR.
Title Transgenic mouse for conditional, tissue-specific Cox-2 overexpression.
Journal Genesis
Abstract We constructed a cyclooxygenase-2 (Cox-2) conditional overexpression transgenic mouse (Cox-2 COE). The transgene contains a CAG promoter driving the Cox-2 and humanized Renilla luciferase (hRL) coding regions, linked by an internal ribosomal entry site. The promoter is followed by a loxP-flanked sequence containing enhanced green fluorescent protein (EGFP), a neomycin selection cassette, and a transcriptional/translational STOP sequence. In the presence of Cre recombinase the loxP-flanked sequence is excised. Cox-2/hRL expression can be monitored repeatedly and noninvasively in vivo by imaging hRL activity. To demonstrate conditional Cox-2 and hRL expression, a nonreplicating adenovirus carrying Cre recombinase (Ad.CMV.Cre) was injected intravenously; hepatic Cox-2 expression and hRL signal were elevated. Cox-2 COE embryonic fibroblasts express both Cox-2 and hRL following Ad.CMV.Cre infection. PGE(2) production is also increased following Ad.CMV.Cre infection of Cox-2 COE embryo fibroblasts. Cox-2 COE mice should be valuable for the study of Cox-2 overexpression in cardiovascular disease, acute and chronic inflammatory responses, neurodegenerative diseases, and cancer.
Volume 44(4)
Pages 177-82
Published 2006-4-1
DOI 10.1002/dvg.20199
PMID 16604526
MeSH Adenoviridae / genetics Animals Cyclooxygenase 2 / analysis Cyclooxygenase 2 / genetics Cyclooxygenase 2 / metabolism* Fibroblasts / enzymology Fibroblasts / metabolism Gene Deletion* Gene Expression Regulation* Genetic Techniques* Genetic Vectors / administration & dosage Green Fluorescent Proteins / metabolism Injections, Intravenous Integrases / genetics Integrases / metabolism Liver / enzymology Liver / metabolism* Luciferases, Renilla / metabolism Mice Mice, Transgenic Models, Genetic Promoter Regions, Genetic Transgenes Viral Proteins / genetics Viral Proteins / metabolism
IF 1.74
Times Cited 13
WOS Category GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY
Resource
DNA material pCALwL (RDB01679) Cox2-COE (RDB05985)