Reference - Detail
| RRC ID | 2441 |
|---|---|
| Author | Watanabe K, Ueno M, Kamiya D, Nishiyama A, Matsumura M, Wataya T, Takahashi JB, Nishikawa S, Nishikawa S, Muguruma K, Sasai Y. |
| Title | A ROCK inhibitor permits survival of dissociated human embryonic stem cells. |
| Journal | Nat Biotechnol |
| Abstract |
Poor survival of human embryonic stem (hES) cells after cell dissociation is an obstacle to research, hindering manipulations such as subcloning. Here we show that application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency (from approximately 1% to approximately 27%) and facilitates subcloning after gene transfer. Furthermore, dissociated hES cells treated with Y-27632 are protected from apoptosis even in serum-free suspension (SFEB) culture and form floating aggregates. We demonstrate that the protective ability of Y-27632 enables SFEB-cultured hES cells to survive and differentiate into Bf1(+) cortical and basal telencephalic progenitors, as do SFEB-cultured mouse ES cells. |
| Volume | 25(6) |
| Pages | 681-6 |
| Published | 2007-6-1 |
| DOI | 10.1038/nbt1310 |
| PII | nbt1310 |
| PMID | 17529971 |
| MeSH | Amides / administration & dosage* Animals Cell Differentiation / drug effects* Cell Survival / drug effects* Cells, Cultured Dose-Response Relationship, Drug Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism* Humans Intracellular Signaling Peptides and Proteins / antagonists & inhibitors* Intracellular Signaling Peptides and Proteins / metabolism* Mice Protein Serine-Threonine Kinases / antagonists & inhibitors* Protein Serine-Threonine Kinases / metabolism* Pyridines / administration & dosage* rho-Associated Kinases |
| IF | 36.553 |
| Times Cited | 1225 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Human embryonic stem cells | KhES-1 KhES-2 KhES-3 |