RRC ID 2452
著者 Carmany-Rampey A, Moens CB.
タイトル Modern mosaic analysis in the zebrafish.
ジャーナル Methods
Abstract One of the most powerful tools used to gain insight into complex developmental processes is the analysis of mosaic embryos. A mosaic is defined as an organism that contains cells of more than one genotype, usually wild-type and mutant. It is the interplay between wild-type and mutant cells in the mosaic that reveals information about the normal function of the mutated gene. Mosaic analysis has been utilized extensively in Caenorhabditis elegans, Drosophila, mice, and zebrafish to elucidate when, where, and how a gene acts during development. In the zebrafish, mosaic analysis has been used to dissect a number of different developmental processes, including gastrulation movements, mesoderm and endoderm specification, neuronal patterning and migration, axon pathfinding, angiogenesis, and cardiac, retinal, and neural crest development. Mosaic analysis is a particularly effective method for understanding gene function in the zebrafish, a model organism particularly suited to forward genetic, molecular, and classical embryological approaches. These attributes, when combined with the accessibility and optical clarity of the zebrafish embryo, facilitate the real time observation of individual cell behaviors and interactions within mosaic embryos.
巻・号 39(3)
ページ 228-38
公開日 2006-7-1
DOI 10.1016/j.ymeth.2006.02.002
PII S1046-2023(06)00064-8
PMID 16829130
MeSH Animals Cell Differentiation / genetics Cell Lineage Cell Transplantation / methods Chimera Embryo, Nonmammalian / cytology Genetic Engineering / methods* Mosaicism* Signal Transduction Zebrafish / genetics* Zebrafish / growth & development Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 3.812
引用数 43
WOS 分野 BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ゼブラフィッシュ rw0