論文 - 詳細
| RRC ID | 2465 |
|---|---|
| 著者 | Cooper KL, Armstrong J, Moens CB. |
| タイトル | Zebrafish foggy/spt 5 is required for migration of facial branchiomotor neurons but not for their survival. |
| ジャーナル | Dev Dyn |
| Abstract |
Transcript elongation is a critical step in the production of mature messenger RNAs. Many factors have been identified that are required for transcript elongation, including Spt 5. Studies in yeast determined that spt 5 is required for cell viability, and analyses in Drosophila indicate Spt 5 is localized to sites of active transcription, suggesting it is required generally for transcription. However, the requirement for spt 5 for cell viability in a metazoan organism has not been addressed. We determined that zebrafish foggy/spt 5 is required cell-autonomously for the posterior migration of facial branchiomotor neurons from rhombomere 4 (r4) into r6 and r7 of the hindbrain. These genetic mosaics also give us the unique opportunity to determine whether spt 5 is required for mRNA transcription equivalently at all loci by addressing two processes within the same cell-neuronal migration and cell viability. In a wild-type host, spt 5 null facial branchiomotor neurons survive to at least 5 days postfertilization while failing to migrate posteriorly. This finding indicates that spt 5-dependent transcript elongation is required cell-autonomously for a complex cell migration but not for the survival of these same cells. This work provides evidence that transcript elongation is not a global mechanism equivalently required by all loci and may actually be under more strict developmental regulation. |
| 巻・号 | 234(3) |
| ページ | 651-8 |
| 公開日 | 2005-11-1 |
| DOI | 10.1002/dvdy.20584 |
| PMID | 16193504 |
| PMC | PMC2597073 |
| MeSH | Animals Base Sequence Cell Movement* Cell Survival DNA-Binding Proteins Face / embryology Gene Expression Regulation, Developmental Molecular Sequence Data Motor Neurons / cytology* Motor Neurons / metabolism* Nuclear Proteins / deficiency Nuclear Proteins / genetics Nuclear Proteins / metabolism* RNA-Binding Proteins Transcription Factors / deficiency Transcription Factors / genetics Transcription Factors / metabolism* Zebrafish / embryology Zebrafish / metabolism* Zebrafish Proteins / deficiency Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 3.275 |
| 引用数 | 14 |
| WOS 分野 | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ゼブラフィッシュ | rw0 |