RRC ID 2489
Author Inoue F, Nagayoshi S, Ota S, Islam ME, Tonou-Fujimori N, Odaira Y, Kawakami K, Yamasu K.
Title Genomic organization, alternative splicing, and multiple regulatory regions of the zebrafish fgf8 gene.
Journal Dev. Growth Differ.
Abstract Fgf8 is among the members of the fibroblast growth factor (FGF) family that play pivotal roles in vertebrate development. In the present study, the genomic DNA of the zebrafish fgf8 gene was cloned to elucidate the regulatory mechanism behind the temporally and spatially restricted expression of the gene in vertebrate embryos. Structural analysis revealed that the exon-intron organization of fgf8 is highly conserved during vertebrate evolution, from teleosts to mammals. Close inspection of the genomic sequence and reverse transcription-polymerase chain reaction analysis revealed that zebrafish fgf8 encodes two splicing variants, corresponding to Fgf8a and Fgf8b, among the four to seven splicing variants known in mammals. Misexpression of the two variants in zebrafish embryos following mRNA injection showed that both variants have dorsalizing activities on zebrafish embryos, with Fgf8b being more potent. Reporter gene analysis of the transcriptional regulation of zebrafish fgf8 suggested that its complicated expression pattern, which is considered essential for its multiple roles in development, is mediated by combinations of different regulatory regions in the upstream and downstream regions of the gene. Furthermore, comparison of the genomic sequence of fgf8 among different vertebrate species suggests that this regulatory mechanism is conserved during vertebrate evolution.
Volume 48(7)
Pages 447-62
Published 2006-9
DOI 10.1111/j.1440-169X.2006.00882.x
PMID 16961592
MeSH Alternative Splicing / genetics* Animals Base Sequence Conserved Sequence / genetics Exons / genetics Fibroblast Growth Factor 8 / genetics* Fibroblast Growth Factor 8 / metabolism Gene Expression Regulation, Developmental Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans In Situ Hybridization Introns / genetics Molecular Sequence Data Protein Isoforms / genetics Protein Isoforms / metabolism Regulatory Sequences, Nucleic Acid / genetics* Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Nucleic Acid Transcription Initiation Site Zebrafish / embryology Zebrafish / genetics* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 1.638
Times Cited 19
Zebrafish Reference