Reference - Detail
|Author||Chen S, Dong X.|
|Title||Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water.|
|Journal||Int. J. Syst. Evol. Microbiol.|
Two mesophilic anaerobic bacterial strains (Z7(T) and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0.2-0.4x4.0-8.0 microm). Growth of the strains was observed at 20-42 degrees C and pH 5.0-7.5. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. The fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids isoC(15 : 0) (42.83 %) and isoC(14 : 0) (32.11 %). The DNA G+C contents of strains Z7(T) and Z1 were 50.4 and 48.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a new phyletic sublineage within the Clostridium leptum rRNA cluster, with <91 % 16S rRNA gene sequence similarity to currently described species. On the basis of polyphasic evidence from this study, Acetanaerobacterium elongatum gen. nov., sp. nov., a novel genus and species, is proposed, with strain Z7(T) (=JCM 12359(T)=AS 1.5012(T)) as the type strain.
|MeSH||Acetic Acid / metabolism Anaerobiosis Base Composition Carbohydrate Metabolism Carbon Dioxide / metabolism China DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Esculin / metabolism Ethanol / metabolism Fatty Acids / chemistry Fatty Acids / isolation & purification Fermentation Gelatin / metabolism Genes, rRNA Gentian Violet Gram-Positive Bacteria / classification* Gram-Positive Bacteria / cytology Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / physiology Hydrogen / metabolism Hydrogen-Ion Concentration Microscopy, Electron Molecular Sequence Data Phenazines Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sewage / microbiology* Spores, Bacterial / cytology Temperature Water Microbiology*|
|Pathogenic microorganisms||JCM 12359?|