RRC ID 2546
Author Chen S, Dong X.
Title Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water.
Journal Int. J. Syst. Evol. Microbiol.
Abstract Two mesophilic anaerobic bacterial strains (Z7(T) and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0.2-0.4x4.0-8.0 microm). Growth of the strains was observed at 20-42 degrees C and pH 5.0-7.5. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. The fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids isoC(15 : 0) (42.83 %) and isoC(14 : 0) (32.11 %). The DNA G+C contents of strains Z7(T) and Z1 were 50.4 and 48.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a new phyletic sublineage within the Clostridium leptum rRNA cluster, with <91 % 16S rRNA gene sequence similarity to currently described species. On the basis of polyphasic evidence from this study, Acetanaerobacterium elongatum gen. nov., sp. nov., a novel genus and species, is proposed, with strain Z7(T) (=JCM 12359(T)=AS 1.5012(T)) as the type strain.
Volume 54(Pt 6)
Pages 2257-62
Published 2004-11
DOI 10.1099/ijs.0.63212-0
PII 54/6/2257
PMID 15545467
MeSH Acetic Acid / metabolism Anaerobiosis Base Composition Carbohydrate Metabolism Carbon Dioxide / metabolism China DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Esculin / metabolism Ethanol / metabolism Fatty Acids / chemistry Fatty Acids / isolation & purification Fermentation Gelatin / metabolism Genes, rRNA Gentian Violet Gram-Positive Bacteria / classification* Gram-Positive Bacteria / cytology Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / physiology Hydrogen / metabolism Hydrogen-Ion Concentration Microscopy, Electron Molecular Sequence Data Phenazines Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sewage / microbiology* Spores, Bacterial / cytology Temperature Water Microbiology*
IF 1.932
Times Cited 38
Pathogenic microorganisms JCM 12359?