論文 - 詳細
| RRC ID | 25673 |
|---|---|
| 著者 | Takenouchi Y, Nakajima H, Kanamaru K, Takumi S. |
| タイトル | Characterization of three homoeologous cDNAs encoding chloroplast-targeted aminolevulinic acid dehydratase in common wheat. |
| ジャーナル | J Integr Plant Biol |
| Abstract |
In the tetrapyrrole biosynthetic pathway of higher plants, 5-aminolevulinic acid (ALA) is metabolized by ALA dehydratase (ALAD). Here, we isolated ALAD1 cDNA from common wheat (Triticum aestivum L.) and its diploid progenitors, and produced transgenic tobacco plants expressing the wheat ALAD1 gene. The ALAD1 genes were highly conserved among wheat relatives, and three homoeologous loci of wheat ALAD1 (TaALAD1) were equally transcribed in common wheat. A transient expression assay of a TaALAD1-GFP (green fluorescent protein) fusion protein suggested that TaALAD1 is localized in chloroplasts. Overexpression of TaALAD1 in transgenic tobacco resulted in a significant increase in ALAD activity in leaves. Moreover, the transgenic tobacco showed vigorous growth and increased survival rate on medium containing ALA at herbicidal concentrations. These results indicate that wheat ALAD1 has catalytic activity in metabolizing ALA in plastids, and that ectopic expression of TaALAD1 in transgenic plants increases their tolerance to ALA application at high concentrations. |
| 巻・号 | 53(12) |
| ページ | 942-50 |
| 公開日 | 2011-12-1 |
| DOI | 10.1111/j.1744-7909.2011.01083.x |
| PMID | 22044778 |
| MeSH | Aminolevulinic Acid / pharmacology Base Sequence China Chloroplasts / drug effects Chloroplasts / enzymology* Chloroplasts / genetics Cloning, Molecular Culture Media / pharmacology DNA, Complementary / genetics* Gene Expression Regulation, Enzymologic / drug effects Gene Expression Regulation, Plant / drug effects Intracellular Space / drug effects Intracellular Space / enzymology Molecular Sequence Data Phylogeny Plant Proteins / genetics* Plant Proteins / metabolism Plants, Genetically Modified Porphobilinogen Synthase / genetics* Porphobilinogen Synthase / metabolism Protein Transport / drug effects Sequence Homology, Nucleic Acid* Tobacco / drug effects Tobacco / genetics Tobacco / growth & development Triticum / drug effects Triticum / enzymology* Triticum / genetics* Triticum / growth & development |
| IF | 4.885 |
| 引用数 | 0 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY PLANT SCIENCES |
| リソース情報 | |
| コムギ | cDNA database |