RRC ID 25677
Author Iehisa JC, Takumi S.
Title Variation in abscisic acid responsiveness of Aegilops tauschii and hexaploid wheat synthetics due to the D-genome diversity.
Journal Genes Genet. Syst.
Abstract Common wheat (Triticum aestivum L.) is an allohexaploid that originated from natural hybridization between tetraploid wheat (Triticum turgidum) and diploid Aegilops tauschii. Ae. tauschii is considered one of the potential sources of new genetic variation in abiotic stress tolerance for improving common wheat. Abscisic acid (ABA) plays an important role in plant adaptation to environmental stresses. In this study, ABA responsiveness of 67 Ae. tauschii accessions and their synthetic hexaploid wheat lines, derived from crosses between T. turgidum cv. Langdon and the Ae. tauschii accessions, was evaluated based on growth inhibition by 20 µM ABA. Wide variation was found in ABA responsiveness for both synthetic wheat lines and their parental Ae. tauschii accessions. The variations due to D-genome found at the diploid level were also expressed in a hexaploid genetic background. Two pairs of synthetic wheat lines differing in ABA responsiveness were then selected for gene expression analysis and to test abiotic stress tolerance, because their parental Ae. tauschii accessions similarly exhibited the differential response to ABA. Gene expression of ABA inducible transcription factor, WABI5, and the downstream Cor/Lea genes (Wrab17, Wdhn13 and Wrab18) were analysed. In one pair, the highly responsive line exhibited higher induction of Wrab17 by ABA treatment, but no significant difference in dehydration or salinity tolerance was observed between these lines. In contrast, in the second pair, the highly ABA-responsive line showed higher levels of Wdhn13 expression and dehydration and salinity tolerance. In synthetic wheat lines, the difference in the ABA responsiveness of the lines appeared to be determined by the different sets of D-genome genes. Our findings suggest that highly ABA-responsive Ae. tauschii accessions should be valuable genetic resources for improving the abiotic stress tolerance of common wheat.
Volume 87(1)
Pages 9-18
Published 2012
PII JST.JSTAGE/ggs/87.9
PMID 22531790
MeSH Abscisic Acid / physiology* Chimera / genetics* Chimera / growth & development Chimera / physiology Dehydration / genetics Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant* Genetic Variation Plant Growth Regulators / physiology* Plant Roots / genetics Plant Roots / growth & development Plant Roots / physiology Ploidies Salt-Tolerance / genetics Seedlings / genetics Seedlings / growth & development Seedlings / physiology Stress, Physiological / genetics Transcription, Genetic Triticum / genetics* Triticum / growth & development Triticum / physiology
IF 0.703
Times Cited 10
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Wheat KU-2022 KU-2059 KU-2810 KU-2811 KU-2814 KU-2816 KU-2824 KU-2069 KU-2074 KU-2075 KU-2076 KU-2078 KU-2079 KU-2080 KU-20-8 KU-20-9 KU-20-10 KU-2083 KU-2088 KU-2090 KU-2092 KU-2093 KU-2096 KU-2097 KU-2098 KU-2100 KU-2101 KU-2102 KU-2104 KU-2105 KU-2106 KU-2108 KU-2109 KU-2111 KU-2124 KU-2126 KU-2144 KU-2145 KU-2152 KU-2155 KU-2156 KU-2157 KU-2158 KU-2159 KU-2160 KU-2132 KU-2136