RRC ID 25679
Author Takumi S, Koyama K, Fujiwara K, Kobayashi F.
Title Identification of a large deletion in the first intron of the Vrn-D1 locus, associated with loss of vernalization requirement in wild wheat progenitor Aegilops tauschii Coss.
Journal Genes Genet. Syst.
Abstract Vernalization promotes flowering in winter wheat cultivars, whereas spring wheat cultivars are able to transition from vegetative to reproductive phase without vernalization. The wheat vernalization requirement is mainly controlled by the major locus Vrn-1, an APETALA1/FRUITFULL MADS-box gene homolog. To study natural variation of the vernalization requirement in a wild progenitor of common wheat, we sequenced the Vrn-D(t)1 locus in four accessions of Aegilops tauschii Coss. Some structural mutations were found in the promoter and first intron regions of Vrn-D(t)1, and haplotype analysis was conducted to examine the distribution of each identified mutation within 211 accessions of Ae. tauschii germplasm. Out of the total, nine accessions, which were originally collected in Afghanistan and Pakistan, contained deletions of a 5.4-kb sequence in the critical region of the Vrn-D(t)1 first intron. The 5.4-kb deletion mutation appeared independently of the dominant allele of the common wheat Vrn-D1 locus. The large deletion was absolutely associated with a lack of vernalization requirement for flowering under long-day conditions, but had no influence on heading date under field growth conditions. The levels of Vrn-1 and WFT transcript increased in the Ae. tauschii accessions having the large deletion. Identification of natural mutant accessions with a loss of vernalization requirement indicates the agricultural significance of Ae. tauschii as a genetic resource for wheat breeding.
Volume 86(3)
Pages 183-95
Published 2011
PII JST.JSTAGE/ggs/86.183
PMID 21952208
MeSH Afghanistan Base Sequence DNA Primers / genetics Flowers / genetics* Flowers / physiology Gene Expression Profiling Genes, Plant / genetics* Haplotypes / genetics Introns / genetics Molecular Sequence Data Pakistan Poaceae / genetics* Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Sequence Deletion / genetics*
IF 0.913
Times Cited 5
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Wheat KU-2010 KU-2012 KU-2016 KU-2018 KU-2022 KU-2025 KU-2027 KU-2028 KU-2032 KU-2035 KU-2039 KU-2042 KU-2043 KU-2044 KU-2050 KU-2051 KU-2056 KU-2058 KU-2059 KU-2061 KU-2063 KU-2066 KU-2612 KU-2616 KU-2617 KU-2619 KU-2621 KU-2624 KU-2627 KU-2630 KU-2632 KU-2633 KU-2635 KU-2636 KU-2638 KU-2639 KU-2809 KU-2810 KU-2811 KU-2814 KU-2816 KU-2821 KU-2822A KU-2823 KU-2824 KU-2801 KU-2804 KU-2806 KU-20-1 KU-2826 KU-2827 KU-2828 KU-2829A KU-2832 KU-2834 KU-2835B KU-2836 KU-2068 KU-2069 KU-20-7 KU-2074 KU-2075 KU-2076 KU-2077 KU-2078 KU-2079 KU-2080 KU-20-8 KU-20-9 KU-20-10 KU-2082 KU-2083 KU-2086 KU-2087 KU-2088 KU-2090 KU-2091 KU-2092 KU-2093 KU-2096 KU-2097 KU-2098 KU-2100 KU-2101 KU-2102 KU-2103 KU-2104 KU-2105 KU-2106 KU-2107 KU-2108 KU-2109 KU-2110 KU-2111 KU-2112 KU-2113 KU-2115 KU-2116 KU-2118 KU-2120 KU-2121 KU-2122 KU-2124 KU-2126 KU-2142 KU-2143 KU-2144 KU-2145 KU-2148 KU-2149 KU-2150 KU-2151 KU-2152 KU-2153 KU-2154 KU-2155 KU-2156 KU-2157 KU-2158 KU-2159 KU-2160 KU-2001 KU-2003 KU-2006 KU-2008 KU-20-6 KU-2131 KU-2132 KU-2133 KU-2136 KU-2137 KU-2138 KU-2140 KU-2141 KU-20-3