RRC ID 260
著者 Yoda H, Hirose Y, Yasuoka A, Sasado T, Morinaga C, Deguchi T, Henrich T, Iwanami N, Watanabe T, Osakada M, Kunimatsu S, Wittbrodt J, Suwa H, Niwa K, Okamoto Y, Yamanaka T, Kondoh H, Furutani-Seiki M.
タイトル Mutations affecting retinotectal axonal pathfinding in Medaka, Oryzias latipes.
ジャーナル Mech Dev
Abstract We screened for mutations affecting retinotectal axonal projection in Medaka, Oryzias latipes. In wild-type Medaka embryos, all the axons of retinal ganglion cells (RGCs) project to the contralateral tectum, such that the topological relationship of the retinal field is maintained. We labeled RGC axons using DiI/DiO at the nasodorsal and temporoventral positions of the retina, and screened for mutations affecting the pattern of stereotypic projections to the tectum. By screening 184 mutagenized haploid genomes, seven mutations in five genes causing defects in axonal pathfinding were identified, whereas mutations affecting the topographic projection of RGC axons were not found. The mutants were grouped into two classes according to their phenotypes. In mutants of Class I, a subpopulation of the RGC axons branched out either immediately after leaving the eye or after reaching the midline, and this axonal subpopulation projected to the ipsilateral tectum. In mutants of Class II, subpopulations of RGC axons branched out after crossing the midline and projected aberrantly. These mutants will provide clues to understanding the functions of genes essential for axonal pathfinding, which may be conserved or partly divergent among vertebrates.
巻・号 121(7-8)
ページ 715-28
公開日 2004-7-1
DOI 10.1016/j.mod.2004.03.021
PII S0925477304000577
PMID 15210179
MeSH Animals Axons* Eye / embryology Mutation* Optic Chiasm / embryology Optic Nerve / abnormalities Optic Nerve / embryology Oryzias / embryology* Oryzias / genetics* Superior Colliculi / embryology Zebrafish / embryology Zebrafish / genetics
IF 2.126
引用数 11
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
メダカ ENU mutagenesis project