Reference - Detail
| RRC ID | 2618 |
|---|---|
| Author | Yumoto I, Hirota K, Nodasaka Y, Yokota Y, Hoshino T, Nakajima K. |
| Title | Alkalibacterium psychrotolerans sp. nov., a psychrotolerant obligate alkaliphile that reduces an indigo dye. |
| Journal | Int J Syst Evol Microbiol |
| Abstract |
A psychrotolerant, obligately alkaliphilic bacterium, IDR2-2(T), which is able to reduce indigo dye was isolated from a fermented polygonum indigo (Polygonum tinctorium Lour.) produced in Date, Hokkaido, using a traditional Japanese method. The isolate grew at pH 9-12 but not at pH 7-8. It was a Gram-positive, facultatively anaerobic, straight rod-shaped bacterium with peritrichous flagella. The isolate grew in 0-17 % (w/v) NaCl but not at NaCl concentrations higher than 18 % (w/v). Its major cellular fatty acids were C(14 : 0), C(16 : 0), C(16 : 1)9c and C(18 : 1)9c, and its DNA G+C content was 40.6 mol%. dl-lactic acid was the major end-product from d-glucose. No quinones could be detected. The peptidoglycan type was A4beta, Orn-d-Glu. A phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain IDR2-2(T) is a member of the genus Alkalibacterium. DNA-DNA hybridization revealed low relatedness (less than 25 %) between the isolate and two phylogenetically related strains, Alkalibacterium olivapovliticus and Marinilactibacillus psychrotolerans. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA relatedness data, the isolate merits classification as a novel species, for which the name Alkalibacterium psychrotolerans sp. nov. is proposed. The type strain is IDR2-2(T) (=JCM 12281(T)=NCIMB 13981(T)). |
| Volume | 54(Pt 6) |
| Pages | 2379-2383 |
| Published | 2004-11-1 |
| DOI | 10.1099/ijs.0.63130-0 |
| PMID | 15545487 |
| MeSH | Anaerobiosis Bacterial Typing Techniques Base Composition Cold Temperature DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Fatty Acids / analysis Fatty Acids / chemistry Fatty Acids / isolation & purification Fermentation Flagella Genes, rRNA Gentian Violet Glucose / metabolism Gram-Positive Bacteria / classification* Gram-Positive Bacteria / cytology Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / metabolism Hydrogen-Ion Concentration Indigo Carmine Indoles / metabolism* Japan Lactic Acid / metabolism Molecular Sequence Data Nucleic Acid Hybridization Oxidation-Reduction Peptidoglycan / chemistry Peptidoglycan / isolation & purification Phenazines Phylogeny Polygonum / microbiology* Quinones / analysis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Saline Solution, Hypertonic Sequence Analysis, DNA |
| IF | 2.415 |
| Times Cited | 56 |
| WOS Category | MICROBIOLOGY |
| Resource | |
| Pathogenic microorganisms | JCM 12281? |