| RRC ID |
27180
|
| 著者 |
Meng Q, Haque A, Hexig B, Akaike T.
|
| タイトル |
The differentiation and isolation of mouse embryonic stem cells toward hepatocytes using galactose-carrying substrata.
|
| ジャーナル |
Biomaterials
|
| Abstract |
A simple culture system to achieve the differentiation of embryonic stem (ES) cells toward hepatocytes with high efficiency is crucial in providing a cell source for the medical application. In this study, we report the effect of a matrix-dependent enrichment of ES cell-derived hepatocytes using immobilized poly(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide) (PVLA) with E-cadherin-IgG Fc (E-cad-Fc) as a galactose-carrying substratum. PVLA and E-cad-Fc were confirmed to be stably co-adsorbed onto polystyrene surface by quartz crystal microbalance (QCM). We showed that the E-cad-Fc/PVLA hybrid substratum was efficient in culturing primary hepatocytes and maintaining liver functions, on which the undifferentiated ES cells also maintained high proliferative capability. Furthermore, ES cell-derived hepatocytes on this hybrid matrix expressed elevated level of liver specific genes and functions together with early expression of definitive hepatocyte marker, asialoglycoprotein receptor (ASGPR). Finally, we isolated a high percentage of cells (about 60%) with ASGPR expression after re-seeding onto PVLA-coated surface, and observed the elimination of the poorly differentiated cells (Gata6(+) and Sox17(+)) and the ones toward another cell lineage (brachyury(+) and Pdx1(+)). The system uses a glycopolymer as an extracellular substratum for isolation and enrichment of ES cell-derived hepatocytes with adequate homogeneity and functionality.
|
| 巻・号 |
33(5)
|
| ページ |
1414-27
|
| 公開日 |
2012-2-1
|
| DOI |
10.1016/j.biomaterials.2011.11.007
|
| PII |
S0142-9612(11)01345-7
|
| PMID |
22118818
|
| MeSH |
Adsorption / drug effects
Animals
Asialoglycoprotein Receptor / metabolism
Cadherins / metabolism
Cell Differentiation / drug effects*
Cell Separation / methods*
Cells, Cultured
Disaccharides / pharmacology*
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / drug effects*
Embryonic Stem Cells / metabolism
Endoderm / cytology
Endoderm / drug effects
Endoderm / metabolism
Galactose / pharmacology*
Hepatocytes / cytology*
Hepatocytes / drug effects
Hepatocytes / metabolism
Immobilized Proteins / metabolism
Mice
Phenotype
Polystyrenes / pharmacology
Receptors, Fc / metabolism
Surface Properties / drug effects
Vinyl Compounds / pharmacology*
|
| IF |
10.317
|
| 引用数 |
25
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| 遺伝子材料 |
Mouse E-cadherin full length cDNA (RDB01184) |
