| RRC ID |
27204
|
| 著者 |
Kurooka H, Kuroda K, Honjo T.
|
| タイトル |
Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
The Notch intracellular region (RAMIC) interacts with a DNA binding protein RBP-J to activate transcription of genes that inhibit cell differentiation. The RAM domain and ankyrin (ANK) repeats of mouse Notch1 RAMIC were shown to be responsible for RBP-J binding and necessary for transactivation. The C-terminal portion of Notch1 RAMIC has also been suggested to be important for transactivation. Using GAL4 fusion constructs, we identified a novel transactivation domain (TAD) between the ANK repeats and the PEST sequence of mouse Notch1. The C-terminal half of mouse Notch2 RAMIC also exhibited TAD activity. Unexpectedly, the RBP-J chimeric protein with the Notch1 TAD failed to activate transcription but the activity was recovered by addition of either the RAM domain or ANK repeats. The results suggest that the activity of Notch1 TAD is repressed by fusion with RBP-J because of the presence of a RBP-J-associated co-repressor(s), which could be displaced by either the RAM domain or ANK repeats. Taken together, mouse Notch1 RAMIC can experimentally be separated into three functional domains: the RAM domain and ANK repeats for RBP-J binding and co-repressor displacement and the C-terminal TAD.
|
| 巻・号 |
26(23)
|
| ページ |
5448-55
|
| 公開日 |
1998-12-1
|
| DOI |
10.1093/nar/26.23.5448
|
| PII |
gkb849
|
| 解説 |
Original paper.
|
| PMID |
9826771
|
| PMC |
PMC147984
|
| MeSH |
3T3 Cells
Animals
Ankyrins / physiology*
COS Cells
Cells, Cultured
DNA-Binding Proteins / genetics
DNA-Binding Proteins / physiology
Immunoglobulin J Recombination Signal Sequence-Binding Protein
Intracellular Fluid / chemistry
Intracellular Fluid / physiology*
Membrane Proteins / antagonists & inhibitors
Membrane Proteins / genetics
Membrane Proteins / physiology*
Mice
Nuclear Proteins*
Receptor, Notch1
Receptors, Cell Surface*
Recombinant Fusion Proteins / pharmacology
Repetitive Sequences, Amino Acid / physiology*
Repressor Proteins / pharmacology
Sequence Deletion
Transcription Factors*
Transcriptional Activation
|
| IF |
11.502
|
| 引用数 |
156
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pGa981-6 (RDB06776) |
