RRC ID 27205
Author Ueda Y, Hijikata M, Takagi S, Takada R, Takada S, Chiba T, Shimotohno K.
Title Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts.
Journal Int J Cancer
Abstract Wnt/beta-catenin signaling plays important roles in tumorigenesis in certain tumors as well as during development. However, the mechanism of tumorigenesis mediated by this signaling remains to be elucidated. We investigated the response of rodent fibroblasts to activation of Wnt/beta-catenin signaling by treatment with conditioned medium containing soluble Wnt-3a protein (W3a-CM) and by expression of a constitutive active beta-catenin gene harbored by an adenovirus vector. W3a-CM induced transcriptional activation of a beta-catenin/T-cell factor (Tcf)-responsive promoter in rodent fibroblasts such as NIH3T3, Rat-1, Swiss3T3 and Balb3T3 cells. In these cells, an increase in saturation density and an inhibition of apoptosis and/or promotion of growth in low-serum medium were induced by treatment with W3a-CM. In Rat-1 cells, morphologic changes were also induced. All these alterations were reversible. Moreover, the inhibition of apoptosis of NIH3T3 cells in low-serum medium and the morphologic changes in Rat-1 cells, but not the increase in saturation density, were also induced by ectopic expression of a constitutive active beta-catenin gene. These results suggested that activation of Wnt/beta-catenin signaling induces inhibition of apoptosis and morphologic changes in these cells.
Volume 99(5)
Pages 681-8
Published 2002-6-10
DOI 10.1002/ijc.10418
Description Original paper.
PMID 12115501
MeSH 3T3 Cells Animals Apoptosis* Binding Sites Cell Division / drug effects Cell Line Culture Media* Culture Media, Conditioned Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* DNA-Binding Proteins / metabolism Fibroblasts / cytology* Gene Expression Immunoblotting Luciferases / genetics Lymphoid Enhancer-Binding Factor 1 Mice Mice, Inbred BALB C Promoter Regions, Genetic Proteins / metabolism Proteins / pharmacology Proto-Oncogene Proteins / metabolism* Rats Recombinant Fusion Proteins Signal Transduction* Trans-Activators* Transcription Factors / metabolism Transfection Wnt Proteins Wnt3 Protein Wnt3A Protein Zebrafish Proteins* beta Catenin
IF 5.145
Times Cited 32
WOS Category ONCOLOGY
Resource
DNA material pTcf7wtluc (RDB02968) pTcf7mtluc (RDB02969)