論文 - 詳細
| RRC ID | 27315 |
|---|---|
| 著者 | Kim KD, Tanizawa H, Iwasaki O, Corcoran CJ, Capizzi JR, Hayden JE, Noma K. |
| タイトル | Centromeric motion facilitates the mobility of interphase genomic regions in fission yeast. |
| ジャーナル | J Cell Sci |
| Abstract |
Dispersed genetic elements, such as retrotransposons and Pol-III-transcribed genes, including tRNA and 5S rRNA, cluster and associate with centromeres in fission yeast through the function of condensin. However, the dynamics of these condensin-mediated genomic associations remains unknown. We have examined the 3D motions of genomic loci including the centromere, telomere, rDNA repeat locus, and the loci carrying Pol-III-transcribed genes or long-terminal repeat (LTR) retrotransposons in live cells at as short as 1.5-second intervals. Treatment with carbendazim (CBZ), a microtubule-destabilizing agent, not only prevents centromeric motion, but also reduces the mobility of the other genomic loci during interphase. Further analyses demonstrate that condensin-mediated associations between centromeres and the genomic loci are clonal, infrequent and transient. However, when associated, centromeres and the genomic loci migrate together in a coordinated fashion. In addition, a condensin mutation that disrupts associations between centromeres and the genomic loci results in a concomitant decrease in the mobility of the loci. Our study suggests that highly mobile centromeres pulled by microtubules in cytoplasm serve as 'genome mobility elements' by facilitating physical relocations of associating genomic regions. |
| 巻・号 | 126(Pt 22) |
| ページ | 5271-83 |
| 公開日 | 2013-11-15 |
| DOI | 10.1242/jcs.133678 |
| PII | jcs.133678 |
| PMID | 23986481 |
| PMC | PMC3828595 |
| MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / ultrastructure Benzimidazoles / pharmacology Carbamates / pharmacology Centromere / genetics* DNA, Ribosomal / genetics DNA, Ribosomal / ultrastructure DNA-Binding Proteins / genetics DNA-Binding Proteins / ultrastructure Genome, Fungal Interphase / genetics* Microtubules / drug effects Microtubules / ultrastructure Mitosis / drug effects Mitosis / genetics* Multiprotein Complexes / genetics Multiprotein Complexes / ultrastructure RNA, Ribosomal, 5S / genetics RNA, Ribosomal, 5S / ultrastructure RNA, Transfer / genetics RNA, Transfer / ultrastructure Retroelements / genetics Schizosaccharomyces / cytology Schizosaccharomyces / genetics* Telomere / genetics Telomere / ultrastructure |
| IF | 4.573 |
| 引用数 | 13 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
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