論文 - 詳細
| RRC ID | 27440 |
|---|---|
| 著者 | Tohno M, Kobayashi H, Nomura M, Kitahara M, Ohkuma M, Uegaki R, Cai Y. |
| タイトル | Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage. |
| ジャーナル | Anim Sci J |
| Abstract |
Twenty-three lactic acid bacteria (LAB) isolated from three cultivars (Akiaoba, Nagahahikari and Tachiwase) of Italian ryegrass (Lolium multiflorum Lam.) silage were precisely characterized by a combination of phenotypic tests, genotypic 16S ribosomal DNA sequencing and rapid PCR-based analyses, focusing on their useful phenotypes for silage preparation as inoculants. We successfully identified both at the species and subspecies levels: phenotypically novel Lactococcus lactis subsp. lactis, Lactobacillus brevis, Lactobacillus coryniformis subsp. torquens, Lactobacillus curvatus, Lactobacillus plantarum subsp. plantarum, Lactobacillus sakei subsp. carnosus, Leuconostoc mesenteroides subsp. dextranicum and Pediococcus parvulus. This is the first report to elucidate the presence of Lactobacillus coryniformis ssp. torquens and Leuconostoc mesenteroides subsp. dextranicum in Italian ryegrass silages. Physiological and biochemical tests revealed that phenotypic characteristics are different among the different strains of the same species and subspecies, and that the isolates show unique and diverse phenotypes related to fermentation factors, such as available carbohydrates, optimal growth pH and temperature. These results suggest that, for various well-preserved silage preparations, the isolates may be useful in producing novel inoculants corresponding to their optimally climatic and ecological niches. |
| 巻・号 | 83(2) |
| ページ | 111-20 |
| 公開日 | 2012-2-1 |
| DOI | 10.1111/j.1740-0929.2011.00923.x |
| PMID | 22339691 |
| MeSH | Fermentation / genetics* Genotype* Hydrogen-Ion Concentration Lactobacillus / genetics* Lactobacillus / isolation & purification* Leuconostoc / genetics Leuconostoc / isolation & purification Lolium / microbiology* Pediococcus / genetics Pediococcus / isolation & purification Phenotype* Polymerase Chain Reaction RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Silage / microbiology* Temperature |
| IF | 1.399 |
| 引用数 | 32 |
| WOS 分野 | AGRICULTURE, DAIRY & ANIMAL SCIENCE |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1059 JCM 1096 JCM 1134 JCM 1149 JCM 1157 JCM 1164 JCM 1166 JCM 1180 JCM 1558 JCM 5805 JCM 5889 JCM 6124 JCM 9700 JCM 10667 JCM 11031 JCM 16167 JCM 16169 JCM 16943 |