論文 - 詳細
| RRC ID | 27492 |
|---|---|
| 著者 | Shinozaki Y, Morita T, Cao XH, Yoshida S, Koitabashi M, Watanabe T, Suzuki K, Sameshima-Yamashita Y, Nakajima-Kambe T, Fujii T, Kitamoto HK. |
| タイトル | Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
Pseudozyma antarctica JCM 10317 exhibits a strong degradation activity for biodegradable plastics (BPs) such as agricultural mulch films composed of poly(butylene succinate) (PBS) and poly(butylene succinate-co-adipate) (PBSA). An enzyme named PaE was isolated and the gene encoding PaE was cloned from the strain by functional complementation in Saccharomyces cerevisiae. The deduced amino acid sequence of PaE contains 198 amino acids with a predicted molecular weight of 20,362.41. High identity was observed between this sequence and that of cutinase-like enzymes (CLEs) (61-68%); therefore, the gene encoding PaE was named PaCLE1. The specific activity of PaE against emulsified PBSA was 54.8±6.3 U/mg. In addition to emulsified BPs, PaE degraded solid films of PBS, PBSA, poly(ε-caprolactone), and poly(lactic acid). |
| 巻・号 | 97(7) |
| ページ | 2951-9 |
| 公開日 | 2013-4-1 |
| DOI | 10.1007/s00253-012-4188-8 |
| PMID | 22678026 |
| MeSH | Adipates / metabolism Amino Acid Sequence Biotransformation Butylene Glycols / metabolism Carboxylic Ester Hydrolases / chemistry Carboxylic Ester Hydrolases / genetics Carboxylic Ester Hydrolases / metabolism* Cloning, Molecular DNA, Fungal / chemistry DNA, Fungal / genetics Molecular Sequence Data Molecular Weight Plastics / metabolism* Polymers / metabolism Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Saccharomyces cerevisiae / genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Succinates / metabolism Ustilaginales / enzymology* Ustilaginales / genetics |
| IF | 3.53 |
| 引用数 | 48 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 10317 |