RRC ID 27541
Author Shojaei H, Daley C, Gitti Z, Hashemi A, Heidarieh P, Moore ER, Naser AD, Russo C, van Ingen J, Tortoli E.
Title Mycobacterium iranicum sp. nov., a rapidly growing scotochromogenic species isolated from clinical specimens on three different continents.
Journal Int. J. Syst. Evol. Microbiol.
Abstract The isolation and characterization of a novel, rapidly growing, scotochromogenic mycobacterial species is reported. Eight independent strains were isolated from clinical specimens from six different countries of the world, two in Iran, two in Italy and one in each of following countries: Greece, The Netherlands, Sweden and the USA. Interestingly, two of the strains were isolated from cerebrospinal fluid. The strains were characterized by rapid growth and presented orange-pigmented scotochromogenic colonies. DNA-based analysis revealed unique sequences in the four regions investigated: the 16S rRNA gene, the rRNA gene internal transcribed spacer 1 and the genes encoding the 65 kDa heat-shock protein and the beta-subunit of RNA polymerase. The phylogenetic analysis placed the strains among the rapidly growing mycobacteria, being most closely related to Mycobacterium gilvum. The genotypic and phenotypic data both strongly supported the inclusion of the strains investigated here as members of a novel species within the genus Mycobacterium; the name Mycobacterium iranicum sp. nov. is proposed to indicate the isolation in Iran of the first recognized strains. The type strain is M05(T) ( = DSM 45541(T) = CCUG 62053(T) = JCM 17461(T)).
Volume 63(Pt 4)
Pages 1383-9
Published 2013-4
DOI 10.1099/ijs.0.043562-0
PII ijs.0.043562-0
PMID 22843713
MeSH Adolescent Adult Aged Aged, 80 and over Bacterial Typing Techniques Cerebrospinal Fluid / microbiology DNA, Bacterial / genetics DNA, Ribosomal Spacer / genetics DNA-Directed RNA Polymerases / genetics Fatty Acids / analysis Female Greece Heat-Shock Proteins / genetics Humans Iran Italy Male Microbial Sensitivity Tests Middle Aged Molecular Sequence Data Mycobacterium / classification* Mycobacterium / genetics Mycobacterium / isolation & purification* Mycobacterium Infections / microbiology Mycolic Acids / analysis Netherlands Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sputum / microbiology Sweden United States Wounds and Injuries / microbiology Young Adult
IF 1.932
Times Cited 12
General Microbes JCM 17461