RRC ID 27594
著者 Krishnamurthi S, Spring S, Kumar PA, Mayilraj S, Klenk HP, Suresh K.
タイトル Desulfotomaculum defluvii sp. nov., a sulfate-reducing bacterium isolated from the subsurface environment of a landfill.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel sulfate-reducing, strictly anaerobic and endospore-forming bacterium, designated strain A5LFS102(T), was isolated from a subsurface landfill sample. The strain was characterized using a polyphasic approach. Optimal growth was observed at 37 °C and pH 7.5 with sulfate as an electron acceptor. Sulfite and thiosulfate were utilized as electron acceptors. The respiratory isoprenoid quinone was menaquinone MK-7. 16S rRNA gene sequence analysis assigned strain A5LFS102(T) to the genus Desulfotomaculum. Both 16S rRNA and dissimilatory sulfate reductase (dsr) genes were compared with those of representative members of the genus Desulfotomaculum. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A5LFS102(T) was closely related to Desulfotomaculum aeronauticum DSM 10349(T) (94.6% sequence similarity). The G+C content of the DNA was 45.4 mol%. The total cellular fatty acid profile was dominated by C16 fatty acids. These phenotypic and genotypic data showed that strain A5LFS102(T) should be recognized as representative of a novel species of the genus Desulfotomaculum, for which the name Desulfotomaculum defluvii sp. nov. is proposed. The type strain is A5LFS102(T) (=DSM 23699(T)=JCM 14036(T)=MTCC 7767(T)).
巻・号 63(Pt 6)
ページ 2290-2295
公開日 2013-6-1
DOI 10.1099/ijs.0.047662-0
PMID 23159750
MeSH Base Composition DNA, Bacterial / genetics Desulfotomaculum / classification* Desulfotomaculum / genetics Desulfotomaculum / isolation & purification Fatty Acids / analysis India Molecular Sequence Data Phylogeny* RNA, Ribosomal, 16S / genetics Refuse Disposal* Sequence Analysis, DNA Sulfates / metabolism Vitamin K 2 / analogs & derivatives Vitamin K 2 / analysis
IF 2.415
引用数 3
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 14036