論文 - 詳細
| RRC ID | 27770 |
|---|---|
| 著者 | Luo SM, Ge ZJ, Wang ZW, Jiang ZZ, Wang ZB, Ouyang YC, Hou Y, Schatten H, Sun QY. |
| タイトル | Unique insights into maternal mitochondrial inheritance in mice. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
In animals, mtDNA is always transmitted through the female and this is termed "maternal inheritance." Recently, autophagy was reported to be involved in maternal inheritance by elimination of paternal mitochondria and mtDNA in Caenorhabditis elegans; moreover, by immunofluorescence, P62 and LC3 proteins were also found to colocalize to sperm mitochondria after fertilization in mice. Thus, it has been speculated that autophagy may be an evolutionary conserved mechanism for paternal mitochondrial elimination. However, by using two transgenic mouse strains, one bearing GFP-labeled autophagosomes and the other bearing red fluorescent protein-labeled mitochondria, we demonstrated that autophagy did not participate in the postfertilization elimination of sperm mitochondria in mice. Although P62 and LC3 proteins congregated to sperm mitochondria immediately after fertilization, sperm mitochondria were not engulfed and ultimately degraded in lysosomes until P62 and LC3 proteins disengaged from sperm mitochondria. Instead, sperm mitochondria unevenly distributed in blastomeres during cleavage and persisted in several cells until the morula stages. Furthermore, by using single sperm mtDNA PCR, we observed that most motile sperm that had reached the oviduct for fertilization had eliminated their mtDNA, leaving only vacuolar mitochondria. However, if sperm with remaining mtDNA entered the zygote, mtDNA was not eliminated and could be detected in newborn mice. Based on these results, we conclude that, in mice, maternal inheritance of mtDNA is not an active process of sperm mitochondrial and mtDNA elimination achieved through autophagy in early embryos, but may be a passive process as a result of prefertilization sperm mtDNA elimination and uneven mitochondrial distribution in embryos. |
| 巻・号 | 110(32) |
| ページ | 13038-43 |
| 公開日 | 2013-8-6 |
| DOI | 10.1073/pnas.1303231110 |
| PII | 1303231110 |
| PMID | 23878233 |
| PMC | PMC3740871 |
| MeSH | Animals Autophagy / genetics* Base Sequence DNA, Mitochondrial / genetics* Embryo, Mammalian / cytology Embryo, Mammalian / embryology Embryo, Mammalian / metabolism Female Genes, Mitochondrial / genetics* Inheritance Patterns / genetics* Luminescent Proteins / genetics Luminescent Proteins / metabolism Lysosomes / metabolism Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Microscopy, Confocal Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Mitochondria / genetics Mitochondria / metabolism Molecular Sequence Data Phagosomes / metabolism Sequence Homology, Nucleic Acid Spermatozoa / cytology Spermatozoa / metabolism Transcription Factor TFIIH Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 9.412 |
| 引用数 | 73 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 RBRC03743 |