RRC ID |
27849
|
著者 |
Weiz AR, Ishida K, Makower K, Ziemert N, Hertweck C, Dittmann E.
|
タイトル |
Leader peptide and a membrane protein scaffold guide the biosynthesis of the tricyclic peptide microviridin.
|
ジャーナル |
Chem Biol
|
Abstract |
Microviridins are unique protease inhibitors from bloom-forming cyanobacteria that have both ecological and pharmacological relevance. Their peptide backbones are produced ribosomally, and ATP grasp ligases introduce ω-ester and ω-amide bonds to yield rare cage-like structures. Bioinformatic analysis of the microviridin biosynthesis gene cluster suggests a novel type of processing machinery, which could rationalize the challenging in vivo/in vitro reconstitution of the pathway. In this work, we report the establishment of a minimal expression system for microviridins. Through bioinformatics and mutational analysis of the MdnA leader peptide we identified and characterized a strictly conserved binding motif that is specific for microviridin ligases. Furthermore, we showed that the ABC transporter MdnE is crucial for cyclization and processing of microviridins and demonstrated that MdnE is essential for stability of the microviridin biosynthesis complex.
|
巻・号 |
18(11)
|
ページ |
1413-21
|
公開日 |
2011-11-23
|
DOI |
10.1016/j.chembiol.2011.09.011
|
PII |
S1074-5521(11)00352-8
|
PMID |
22118675
|
MeSH |
Amino Acid Motifs
Amino Acid Sequence
Bacterial Proteins / metabolism
Cyanobacteria / enzymology
Cyanobacteria / metabolism
Escherichia coli / metabolism
Ligases / metabolism
Membrane Proteins / metabolism*
Molecular Sequence Data
Mutation
Peptides, Cyclic / biosynthesis*
Peptides, Cyclic / chemistry
Peptides, Cyclic / genetics
Protein Sorting Signals*
Sequence Alignment
|
IF |
5.915
|
引用数 |
24
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
藻類 |
NIES-843
NIES-298 |