論文 - 詳細
| RRC ID | 27884 |
|---|---|
| 著者 | Alirezaei M, Kemball CC, Flynn CT, Wood MR, Whitton JL, Kiosses WB. |
| タイトル | Short-term fasting induces profound neuronal autophagy. |
| ジャーナル | Autophagy |
| Abstract |
Disruption of autophagy--a key homeostatic process in which cytosolic components are degraded and recycled through lysosomes--can cause neurodegeneration in tissue culture and in vivo. Upregulation of this pathway may be neuroprotective, and much effort is being invested in developing drugs that cross the blood brain barrier and increase neuronal autophagy. One well-recognized way of inducing autophagy is by food restriction, which upregulates autophagy in many organs including the liver; but current dogma holds that the brain escapes this effect, perhaps because it is a metabolically privileged site. Here, we have re-evaluated this tenet using a novel approach that allows us to detect, enumerate and characterize autophagosomes in vivo. We first validate the approach by showing that it allows the identification and characterization of autophagosomes in the livers of food-restricted mice. We use the method to identify constitutive autophagosomes in cortical neurons and Purkinje cells, and we show that short-term fasting leads to a dramatic upregulation in neuronal autophagy. The increased neuronal autophagy is revealed by changes in autophagosome abundance and characteristics, and by diminished neuronal mTOR activity in vivo, demonstrated by a reduction in levels of phosphorylated S6 ribosomal protein in Purkinje cells. The increased abundance of autophagosomes in Purkinje cells was confirmed using transmission electron microscopy. Our data lead us to speculate that sporadic fasting might represent a simple, safe and inexpensive means to promote this potentially therapeutic neuronal response. |
| 巻・号 | 6(6) |
| ページ | 702-10 |
| 公開日 | 2010-8-1 |
| DOI | 10.4161/auto.6.6.12376 |
| PII | 12376 |
| PMID | 20534972 |
| PMC | PMC3106288 |
| MeSH | Animals Autophagy / physiology* Caloric Restriction Cerebellum / cytology Cerebellum / ultrastructure Cerebral Cortex / cytology Cerebral Cortex / metabolism Cerebral Cortex / ultrastructure Fasting / physiology* Liver / cytology Liver / metabolism Mice Mice, Inbred C57BL Microscopy, Confocal Neurons / cytology* Phagosomes / metabolism Phagosomes / ultrastructure Purkinje Cells / cytology Purkinje Cells / metabolism Purkinje Cells / ultrastructure Reproducibility of Results TOR Serine-Threonine Kinases / metabolism Time Factors |
| IF | 9.77 |
| 引用数 | 116 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1339 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 15.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |