論文 - 詳細
| RRC ID | 2790 |
|---|---|
| 著者 | Sakurai-Yamashita Y, Shigematsu K, Yamashita K, Niwa M. |
| タイトル | Expression of MCP-1 in the hippocampus of SHRSP with ischemia-related delayed neuronal death. |
| ジャーナル | Cell Mol Neurobiol |
| Abstract |
1. The expression of monocyte chemoattractant protein-1 (MCP-1) was examined in stroke-prone spontaneously hypertensive rats with transient global ischemia in order to study the involvement of the infiltration of blood monocytes in the mechanism of ischemia-related neuronal death. 2. The brains of the animals with occlusion of the bilateral carotid arteries for 10 min were removed at 8 h, 1, 2, 4 and 7 days after reperfusion. Frozen sections were used for in situ hybridization and tissue specimens from the hippocampus and the cerebral cortex were used to measure the concentration of MCP-1 by ELISA. 3. No MCP-1 mRNA was detected in the hippocampus of the sham group animals. One day after ischemia-reperfusion, MCP-1 mRNA was clearly expressed in the CA4 subfield and the molecular layer of the dentate gyrus, while it was slightly expressed in the lacnosum moleculare of the CA1 subfield. A dramatic expression was demonstrated in the entire CA1 subfield at 2 days after the operation. Most of the cells expressing MCP-1 were astrocytes. At 4 and 7 days after reperfusion, no MCP-1 mRNA was detected in the hippocampus. The concentration of MCP-1 protein dramatically increased in the hippocampus at 2 days after reperfusion. 4. Taken together with the findings of our previous study showing an increased permeability of the blood-brain barrier in the hippocampus from 12 h after ischemia-reperfusion, the astrocytes expressing MCP-1 might therefore induce the migration of monocytes into the brain parenchyma. As a result, such astrocytes expressing MCP-1 may therefore be related to the pathological events of delayed neuronal death in the pyramidal neurons. |
| 巻・号 | 26(4-6) |
| ページ | 823-31 |
| 公開日 | 2006-1-1 |
| DOI | 10.1007/s10571-006-9077-1 |
| PMID | 16758320 |
| MeSH | Animals Brain Ischemia / metabolism Brain Ischemia / pathology* Carotid Artery, Common / pathology Cell Death Chemokine CCL2 / metabolism* Hippocampus / blood supply Hippocampus / metabolism* Hippocampus / pathology Male Neurons / metabolism Neurons / pathology* Neurons / physiology Rats Rats, Inbred SHR Stroke / complications Stroke / metabolism |
| IF | 3.606 |
| 引用数 | 24 |
| WOS 分野 | NEUROSCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ラット | SHRSP/Ngsk(strainID=303) |