| RRC ID |
2790
|
| 著者 |
Sakurai-Yamashita Y, Shigematsu K, Yamashita K, Niwa M.
|
| タイトル |
Expression of MCP-1 in the hippocampus of SHRSP with ischemia-related delayed neuronal death.
|
| ジャーナル |
Cell Mol Neurobiol
|
| Abstract |
1. The expression of monocyte chemoattractant protein-1 (MCP-1) was examined in stroke-prone spontaneously hypertensive rats with transient global ischemia in order to study the involvement of the infiltration of blood monocytes in the mechanism of ischemia-related neuronal death. 2. The brains of the animals with occlusion of the bilateral carotid arteries for 10 min were removed at 8 h, 1, 2, 4 and 7 days after reperfusion. Frozen sections were used for in situ hybridization and tissue specimens from the hippocampus and the cerebral cortex were used to measure the concentration of MCP-1 by ELISA. 3. No MCP-1 mRNA was detected in the hippocampus of the sham group animals. One day after ischemia-reperfusion, MCP-1 mRNA was clearly expressed in the CA4 subfield and the molecular layer of the dentate gyrus, while it was slightly expressed in the lacnosum moleculare of the CA1 subfield. A dramatic expression was demonstrated in the entire CA1 subfield at 2 days after the operation. Most of the cells expressing MCP-1 were astrocytes. At 4 and 7 days after reperfusion, no MCP-1 mRNA was detected in the hippocampus. The concentration of MCP-1 protein dramatically increased in the hippocampus at 2 days after reperfusion. 4. Taken together with the findings of our previous study showing an increased permeability of the blood-brain barrier in the hippocampus from 12 h after ischemia-reperfusion, the astrocytes expressing MCP-1 might therefore induce the migration of monocytes into the brain parenchyma. As a result, such astrocytes expressing MCP-1 may therefore be related to the pathological events of delayed neuronal death in the pyramidal neurons.
|
| 巻・号 |
26(4-6)
|
| ページ |
823-31
|
| 公開日 |
2006-1-1
|
| DOI |
10.1007/s10571-006-9077-1
|
| PMID |
16758320
|
| MeSH |
Animals
Brain Ischemia / metabolism
Brain Ischemia / pathology*
Carotid Artery, Common / pathology
Cell Death
Chemokine CCL2 / metabolism*
Hippocampus / blood supply
Hippocampus / metabolism*
Hippocampus / pathology
Male
Neurons / metabolism
Neurons / pathology*
Neurons / physiology
Rats
Rats, Inbred SHR
Stroke / complications
Stroke / metabolism
|
| IF |
3.606
|
| 引用数 |
24
|
|
WOS 分野
|
NEUROSCIENCES
CELL BIOLOGY
|
| リソース情報 |
| ラット |
SHRSP/Ngsk(strainID=303) |
