RRC ID 27917
Author Svensk E, Ståhlman M, Andersson CH, Johansson M, Borén J, Pilon M.
Title PAQR-2 regulates fatty acid desaturation during cold adaptation in C. elegans.
Journal PLoS Genet
Abstract C. elegans PAQR-2 is homologous to the insulin-sensitizing adiponectin receptors in mammals, and essential for adaptation to growth at 15°C, a low but usually acceptable temperature for this organism. By screening for novel paqr-2 suppressors, we identified mutations in genes involved in phosphatidylcholine synthesis (cept-1, pcyt-1 and sams-1) and fatty acid metabolism (ech-7, hacd-1, mdt-15, nhr-49 and sbp-1). We then show genetic evidence that paqr-2, phosphatidylcholines, sbp-1 and Δ9-desaturases form a cold adaptation pathway that regulates the increase in unsaturated fatty acids necessary to retain membrane fluidity at low temperatures. This model is supported by the observations that the paqr-2 suppressors normalize the levels of saturated fatty acids, and that low concentrations of detergents that increase membrane fluidity can rescue the paqr-2 mutant.
Volume 9(9)
Pages e1003801
Published 2013-1-1
DOI 10.1371/journal.pgen.1003801
PMID 24068966
PMC PMC3772066
MeSH Adaptation, Physiological / genetics* Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics* Cold Temperature Fatty Acids / chemistry Fatty Acids / metabolism* Insulin / metabolism Lipid Metabolism / genetics* Membrane Proteins / genetics* Mutation Phosphatidylcholines / metabolism Receptors, Adiponectin / genetics* Receptors, Adiponectin / metabolism Temperature
IF 5.175
Times Cited 44
C.elegans tm3410 tm3262 tm1011