| RRC ID |
2794
|
| 著者 |
Suzuki T, Mandai M, Akimoto M, Yoshimura N, Takahashi M.
|
| タイトル |
The simultaneous treatment of MMP-2 stimulants in retinal transplantation enhances grafted cell migration into the host retina.
|
| ジャーナル |
Stem Cells
|
| Abstract |
The success of functional retinal cell transplantation has been limited by the low efficiency of the transplanted cell integration into the host retina. Given that the extracellular matrix (ECM) is thought to inhibit entry and axonal outgrowth of grafted neural cells into the host retina, modulation of the ECMs in the host environment may overcome this limitation. Here, we demonstrate that matrix metalloprotease-2 (MMP-2) expression is associated with the high migratory potential of adult rat hippocampus-derived neural stem cells compared with retinal progenitor cells. In addition, MMP-2, as well as its reported inducers concanavalin A and 17beta-estradiol, can trigger the migration of retinal progenitor cells into explanted retinas. Inhibitors of MMP-2 suppressed these effects. Intense cell migration is not required for photoreceptor transplantation; however, the environment that allows the transplanted cells to integrate is most important. Migration of the transplanted cells is a good index of the acceptance of grafted cell of the host tissue. Strategies modulating the environment by MMP-2 stimulation may provide an advance in the development of retinal transplantation.
|
| 巻・号 |
24(11)
|
| ページ |
2406-11
|
| 公開日 |
2006-11-1
|
| DOI |
10.1634/stemcells.2005-0587
|
| PII |
24/11/2406
|
| PMID |
17071857
|
| MeSH |
Adult Stem Cells / drug effects
Adult Stem Cells / enzymology*
Adult Stem Cells / transplantation
Animals
Cell Movement* / drug effects
Cells, Cultured
Concanavalin A / pharmacology
Enzyme Activation / drug effects
Enzyme Inhibitors / pharmacology
Estradiol / pharmacology
Hippocampus / cytology
Hippocampus / enzymology
Matrix Metalloproteinase 2 / genetics
Matrix Metalloproteinase 2 / metabolism*
Matrix Metalloproteinase 2 / pharmacology
Matrix Metalloproteinase Inhibitors
Mice
Mice, Inbred C57BL
Neurons / transplantation
Organ Culture Techniques
Photoreceptor Cells / enzymology
Photoreceptor Cells / transplantation*
RNA, Messenger / metabolism
Rats
Rats, Inbred F344
Retina / cytology
Retina / drug effects
Retina / enzymology*
Stem Cell Transplantation*
Transcription, Genetic / drug effects
|
| IF |
6.022
|
| 引用数 |
40
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
HEMATOLOGY
ONCOLOGY
CELL BIOLOGY
CELL & TISSUE ENGINEERING
|
| リソース情報 |
| ラット |
|
