RRC ID 2794
Author Suzuki T, Mandai M, Akimoto M, Yoshimura N, Takahashi M.
Title The simultaneous treatment of MMP-2 stimulants in retinal transplantation enhances grafted cell migration into the host retina.
Journal Stem Cells
Abstract The success of functional retinal cell transplantation has been limited by the low efficiency of the transplanted cell integration into the host retina. Given that the extracellular matrix (ECM) is thought to inhibit entry and axonal outgrowth of grafted neural cells into the host retina, modulation of the ECMs in the host environment may overcome this limitation. Here, we demonstrate that matrix metalloprotease-2 (MMP-2) expression is associated with the high migratory potential of adult rat hippocampus-derived neural stem cells compared with retinal progenitor cells. In addition, MMP-2, as well as its reported inducers concanavalin A and 17beta-estradiol, can trigger the migration of retinal progenitor cells into explanted retinas. Inhibitors of MMP-2 suppressed these effects. Intense cell migration is not required for photoreceptor transplantation; however, the environment that allows the transplanted cells to integrate is most important. Migration of the transplanted cells is a good index of the acceptance of grafted cell of the host tissue. Strategies modulating the environment by MMP-2 stimulation may provide an advance in the development of retinal transplantation.
Volume 24(11)
Pages 2406-11
Published 2006-11
DOI 10.1634/stemcells.2005-0587
PII 24/11/2406
PMID 17071857
MeSH Adult Stem Cells / drug effects Adult Stem Cells / enzymology* Adult Stem Cells / transplantation Animals Cell Movement* / drug effects Cells, Cultured Concanavalin A / pharmacology Enzyme Activation / drug effects Enzyme Inhibitors / pharmacology Estradiol / pharmacology Hippocampus / cytology Hippocampus / enzymology Matrix Metalloproteinase 2 / genetics Matrix Metalloproteinase 2 / metabolism* Matrix Metalloproteinase 2 / pharmacology Matrix Metalloproteinase Inhibitors Mice Mice, Inbred C57BL Neurons / transplantation Organ Culture Techniques Photoreceptor Cells / enzymology Photoreceptor Cells / transplantation* RNA, Messenger / metabolism Rats Rats, Inbred F344 Retina / cytology Retina / drug effects Retina / enzymology* Stem Cell Transplantation* Transcription, Genetic / drug effects
IF 5.614
Times Cited 36