Reference - Detail
|Author||Takahashi M, Haruta M.|
|Title||Derivation and characterization of lentoid bodies and retinal pigment epithelial cells from monkey embryonic stem cells in vitro.|
|Journal||Methods Mol. Biol.|
For cell replacement therapies of retinal diseases, the most-needed cells are retinal pigment epithelial cells and photoreceptor cells; lens cells are needed for replacement of the cataract. Stromal cell-derived inducing activity induces the differentiation of mouse embryonic stem (ES) cells into neural cells, including midbrain tyrosine hydroxylase-positive dopaminergic neurons, and ocular cells; the method also works with primate ES cells. We describe the methods to induce retinal pigment epithelial and lens cells from monkey ES cells using stromal cell-derived inducing activity and show the characteristics of those cells in vitro and in vivo.
|MeSH||Animals Behavior, Animal / physiology Cell Culture Techniques* Cell Differentiation / physiology* Cell Transplantation Cells, Cultured Embryo, Mammalian / cytology Epithelial Cells / cytology Epithelial Cells / physiology* Macaca fascicularis Mice Pigment Epithelium of Eye / cytology* Rats Stem Cells / cytology Stem Cells / physiology*|