| RRC ID |
2806
|
| 著者 |
Veszelka S, Pásztói M, Farkas AE, Krizbai I, Ngo TK, Niwa M, Abrahám CS, Deli MA.
|
| タイトル |
Pentosan polysulfate protects brain endothelial cells against bacterial lipopolysaccharide-induced damages.
|
| ジャーナル |
Neurochem Int
|
| Abstract |
Peripheral inflammation can aggravate local brain inflammation and neuronal death. The blood-brain barrier (BBB) is a key player in the event. On a relevant in vitro model of primary rat brain endothelial cells co-cultured with primary rat astroglia cells lipopolysaccharide (LPS)-induced changes in several BBB functions have been investigated. LPS-treatment resulted in a dose- and time-dependent decrease in the integrity of endothelial monolayers: transendothelial electrical resistance dropped, while flux of permeability markers fluorescein and albumin significantly increased. Immunostaining for junctional proteins ZO-1, claudin-5 and beta-catenin was significantly weaker in LPS-treated endothelial cells than in control monolayers. LPS also reduced the intensity and changed the pattern of ZO-1 immunostaining in freshly isolated rat brain microvessels. The activity of P-glycoprotein, an important efflux pump at the BBB, was also inhibited by LPS. At the same time production of reactive oxygen species and nitric oxide was increased in brain endothelial cells treated with LPS. Pentosan polysulfate, a polyanionic polysaccharide could reduce the deleterious effects of LPS on BBB permeability, and P-glycoprotein activity. LPS-stimulated increase in the production of reactive oxygen species and nitric oxide was also decreased by pentosan treatment. The protective effect of pentosan for brain endothelium can be of therapeutical significance in bacterial infections affecting the BBB.
|
| 巻・号 |
50(1)
|
| ページ |
219-28
|
| 公開日 |
2007-1-1
|
| DOI |
10.1016/j.neuint.2006.08.006
|
| PII |
S0197-0186(06)00267-1
|
| PMID |
16997427
|
| MeSH |
ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
Animals
Blood-Brain Barrier
Brain / blood supply
Brain / drug effects*
Endothelium, Vascular / cytology
Endothelium, Vascular / drug effects*
Lipopolysaccharides / pharmacology*
Membrane Potentials / drug effects
Pentosan Sulfuric Polyester / pharmacokinetics
Pentosan Sulfuric Polyester / pharmacology*
Rats
|
| IF |
3.881
|
| 引用数 |
68
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
NEUROSCIENCES
|
| リソース情報 |
| ラット |
|
