論文 - 詳細
| RRC ID | 28065 |
|---|---|
| 著者 | Arefin B, Kucerova L, Dobes P, Markus R, Strnad H, Wang Z, Hyrsl P, Zurovec M, Theopold U. |
| タイトル | Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins. |
| ジャーナル | J Innate Immun |
| Abstract |
Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses. |
| 巻・号 | 6(2) |
| ページ | 192-204 |
| 公開日 | 2013-8-27 |
| DOI | 10.1159/000353734 |
| PII | 000353734 |
| PMID | 23988573 |
| PMC | PMC6741570 |
| MeSH | Animals Animals, Genetically Modified Cluster Analysis Complement System Proteins / genetics Complement System Proteins / immunology* Complement System Proteins / metabolism Drosophila Proteins / genetics Drosophila Proteins / immunology Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / immunology* Drosophila melanogaster / parasitology Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / immunology* Extracellular Matrix Proteins / metabolism Gene Ontology Genome, Insect* Hemocytes / immunology Hemocytes / metabolism Host-Parasite Interactions / immunology Host-Pathogen Interactions / immunology Larva / genetics Larva / immunology Larva / parasitology Luminescent Proteins / genetics Luminescent Proteins / metabolism Membrane Glycoproteins / genetics Membrane Glycoproteins / immunology Membrane Glycoproteins / metabolism Microscopy, Confocal Oligonucleotide Array Sequence Analysis Photorhabdus / immunology Photorhabdus / physiology RNA Interference Reverse Transcriptase Polymerase Chain Reaction Rhabditoidea / immunology* Rhabditoidea / microbiology Rhabditoidea / physiology Transcriptome / immunology* |
| IF | 4.932 |
| 引用数 | 57 |
| WOS 分野 | IMMUNOLOGY |
| リソース情報 | |
| ショウジョウバエ | 13422R-3 |