RRC ID 28068
著者 Jin Y, Xu J, Yin MX, Lu Y, Hu L, Li P, Zhang P, Yuan Z, Ho MS, Ji H, Zhao Y, Zhang L.
タイトル Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signaling.
ジャーナル Elife
Abstract Chromatin remodeling processes are among the most important regulatory mechanisms in controlling cell proliferation and regeneration. Drosophila intestinal stem cells (ISCs) exhibit self-renewal potentials, maintain tissue homeostasis, and serve as an excellent model for studying cell growth and regeneration. In this study, we show that Brahma (Brm) chromatin-remodeling complex is required for ISC proliferation and damage-induced midgut regeneration in a lineage-specific manner. ISCs and enteroblasts exhibit high levels of Brm proteins; and without Brm, ISC proliferation and differentiation are impaired. Importantly, the Brm complex participates in ISC proliferation induced by the Scalloped-Yorkie transcriptional complex and that the Hippo (Hpo) signaling pathway directly restricted ISC proliferation by regulating Brm protein levels by inducing caspase-dependent cleavage of Brm. The cleavage resistant form of Brm protein promoted ISC proliferation. Our findings highlighted the importance of Hpo signaling in regulating epigenetic components such as Brm to control downstream transcription and hence ISC proliferation. DOI:http://dx.doi.org/10.7554/eLife.00999.001.
巻・号 2
ページ e00999
公開日 2013-10-15
DOI 10.7554/eLife.00999
PII 00999
PMID 24137538
PMC PMC3796317
MeSH Animals Caspases / metabolism Cell Cycle Proteins / metabolism Cell Cycle Proteins / physiology* Cell Differentiation / physiology Cell Proliferation* Drosophila Proteins / metabolism* Drosophila Proteins / physiology* Enzyme Activation Intestines / cytology* Intracellular Signaling Peptides and Proteins / metabolism* Protein Serine-Threonine Kinases / metabolism* Proteolysis Signal Transduction / physiology* Stem Cells / cytology* Trans-Activators / metabolism Trans-Activators / physiology*
IF 7.08
引用数 39
WOS 分野 BIOLOGY
リソース情報
ショウジョウバエ 4303R-1