| RRC ID |
28072
|
| 著者 |
Neumüller RA, Gross T, Samsonova AA, Vinayagam A, Buckner M, Founk K, Hu Y, Sharifpoor S, Rosebrock AP, Andrews B, Winston F, Perrimon N.
|
| タイトル |
Conserved regulators of nucleolar size revealed by global phenotypic analyses.
|
| ジャーナル |
Sci Signal
|
| Abstract |
Regulation of cell growth is a fundamental process in development and disease that integrates a vast array of extra- and intracellular information. A central player in this process is RNA polymerase I (Pol I), which transcribes ribosomal RNA (rRNA) genes in the nucleolus. Rapidly growing cancer cells are characterized by increased Pol I-mediated transcription and, consequently, nucleolar hypertrophy. To map the genetic network underlying the regulation of nucleolar size and of Pol I-mediated transcription, we performed comparative, genome-wide loss-of-function analyses of nucleolar size in Saccharomyces cerevisiae and Drosophila melanogaster coupled with mass spectrometry-based analyses of the ribosomal DNA (rDNA) promoter. With this approach, we identified a set of conserved and nonconserved molecular complexes that control nucleolar size. Furthermore, we characterized a direct role of the histone information regulator (HIR) complex in repressing rRNA transcription in yeast. Our study provides a full-genome, cross-species analysis of a nuclear subcompartment and shows that this approach can identify conserved molecular modules.
|
| 巻・号 |
6(289)
|
| ページ |
ra70
|
| 公開日 |
2013-8-20
|
| DOI |
10.1126/scisignal.2004145
|
| PII |
6/289/ra70
|
| PMID |
23962978
|
| PMC |
PMC3964804
|
| MeSH |
Cell Nucleolus / genetics
Cell Nucleolus / metabolism*
DNA, Fungal / genetics
DNA, Fungal / metabolism
DNA, Ribosomal / genetics
DNA, Ribosomal / metabolism
Genes, Fungal / physiology
Genes, rRNA / physiology
Histones / genetics
Histones / metabolism
RNA Polymerase I / genetics
RNA Polymerase I / metabolism*
RNA, Fungal / biosynthesis*
RNA, Fungal / genetics
RNA, Ribosomal / biosynthesis*
RNA, Ribosomal / genetics
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae / physiology*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*
Transcription, Genetic / physiology*
|
| IF |
6.467
|
| 引用数 |
39
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
1828R-2
1828R-3
4817R-2 |
