RRC ID 28093
Author Suzuki H, Ito R, Ikeda K, Tamura TA.
Title TATA-binding protein (TBP)-like protein is required for p53-dependent transcriptional activation of upstream promoter of p21Waf1/Cip1 gene.
Journal J Biol Chem
Abstract TATA-binding protein-like protein (TLP) is involved in development, checkpoint, and apoptosis through potentiation of gene expression. TLP-overexpressing human cells, especially p53-containing cells, exhibited a decreased growth rate and increased proportion of G(1) phase cells. TLP stimulated expression of several growth-related genes including p21 (p21(Waf1/Cip1)). TLP-mediated activation of the p21 upstream promoter in cells was shown by a promoter-luciferase reporter assay. The p53-binding sequence located in the p21 upstream promoter and p53 itself are required for TLP-mediated transcriptional activation. TLP and p53 bound to each other and synergistically enhanced activity of the upstream promoter. TLP specifically activated transcription from the endogenous upstream promoter, and p53 was required for this activation. Etoposide treatment also resulted in activation of the upstream promoter as well as nuclear accumulation of TLP and p53. Moreover, the upstream promoter was associated with endogenous p53 and TLP, and the p53 recruitment was enhanced by TLP. The results of the present study suggest that TLP mediates p53-governed transcriptional activation of the p21 upstream promoter.
Volume 287(24)
Pages 19792-803
Published 2012-6-8
DOI 10.1074/jbc.M112.369629
PII M112.369629
PMID 22511763
PMC PMC3370165
MeSH Animals Antineoplastic Agents, Phytogenic / pharmacology COS Cells Chlorocebus aethiops Cyclin-Dependent Kinase Inhibitor p21 / biosynthesis* Cyclin-Dependent Kinase Inhibitor p21 / genetics Etoposide / pharmacology G1 Phase / drug effects G1 Phase / physiology HeLa Cells Hep G2 Cells Humans Response Elements / physiology* TATA Box Binding Protein-Like Proteins / genetics TATA Box Binding Protein-Like Proteins / metabolism* Transcription, Genetic / drug effects Transcription, Genetic / physiology* Transcriptional Activation / drug effects Transcriptional Activation / physiology* Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism*
IF 4.106
Times Cited 15
DNA material pKM2L-phP21 (RDB05893)