論文 - 詳細
| RRC ID | 28094 |
|---|---|
| 著者 | Yu Z, Wu H, Chen H, Wang R, Liang X, Liu J, Li C, Deng WM, Jiao R. |
| タイトル | CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development. |
| ジャーナル | Development |
| Abstract |
The histone chaperone CAF-1 is known for its role in DNA replication-coupled histone deposition. However, loss of function causes lethality only in higher multicellular organisms such as mice and flies, but not in unicellular organisms such as yeasts, suggesting that CAF-1 has other important functions than histone deposition during animal development. Emerging evidence indicates that CAF-1 also has a role in higher order chromatin organization and heterochromatin-mediated gene expression; it remains unclear whether CAF-1 has a role in specific signaling cascades to promote gene expression during development. Here, we report that knockdown of one of the subunits of Drosophila CAF-1, dCAF-1-p105 (Caf1-105), results in phenotypes that resemble those of, and are augmented synergistically by, mutations of Notch positive regulatory pathway components. Depletion of dCAF-1-p105 leads to abrogation of cut expression and to downregulation of other Notch target genes in wing imaginal discs. dCAF-1-p105 is associated with Suppressor of Hairless [Su(H)] and regulates its binding to the enhancer region of E(spl)mβ. The association of dCAF-1-p105 with Su(H) on chromatin establishes an active local chromatin status for transcription by maintaining a high level of histone H4 acetylation. In response to induced Notch activation, dCAF-1 associates with the Notch intracellular domain to activate the expression of Notch target genes in cultured S2 cells, manifesting the role of dCAF-1 in Notch signaling. Together, our results reveal a novel epigenetic function of dCAF-1 in promoting Notch pathway activity that regulates normal Drosophila development. |
| 巻・号 | 140(17) |
| ページ | 3635-44 |
| 公開日 | 2013-9-1 |
| DOI | 10.1242/dev.094599 |
| PII | 140/17/3635 |
| PMID | 23942516 |
| PMC | PMC3742146 |
| MeSH | Animals Blotting, Western Chromatin Immunoprecipitation DNA Primers / genetics Drosophila / embryology* Drosophila Proteins / metabolism* Epigenesis, Genetic / physiology* Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology* Homeodomain Proteins / metabolism Imaginal Discs / metabolism Immunohistochemistry Immunoprecipitation Nuclear Proteins / metabolism RNA Interference Receptors, Notch / metabolism* Repressor Proteins / metabolism Retinoblastoma-Binding Protein 4 / metabolism* Signal Transduction / genetics* Transcription Factors / metabolism |
| IF | 5.611 |
| 引用数 | 19 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 12892R-1 12892R-3 |