Reference - Detail
| RRC ID | 28109 |
|---|---|
| Author | Singh P, Kuo YC, Mishra S, Tsai CH, Chien CC, Chen CW, Desclos-Theveniau M, Chu PW, Schulze B, Chinchilla D, Boller T, Zimmerli L. |
| Title | The lectin receptor kinase-VI.2 is required for priming and positively regulates Arabidopsis pattern-triggered immunity. |
| Journal | Plant Cell |
| Abstract |
Plant cells can be sensitized toward a subsequent pathogen attack by avirulent pathogens or by chemicals such as β-aminobutyric acid (BABA). This process is called priming. Using a reverse genetic approach in Arabidopsis thaliana, we demonstrate that the BABA-responsive L-type lectin receptor kinase-VI.2 (LecRK-VI.2) contributes to disease resistance against the hemibiotrophic Pseudomonas syringae and the necrotrophic Pectobacterium carotovorum bacteria. Accordingly, LecRK-VI.2 mRNA levels increased after bacterial inoculation or treatments with microbe-associated molecular patterns (MAMPs). We also show that LecRK-VI.2 is required for full activation of pattern-triggered immunity (PTI); notably, lecrk-VI.2-1 mutants show reduced upregulation of PTI marker genes, impaired callose deposition, and defective stomatal closure. Overexpression studies combined with genome-wide microarray analyses indicate that LecRK-VI.2 positively regulates the PTI response. LecRK-VI.2 is demonstrated to act upstream of mitogen-activated protein kinase signaling, but independently of reactive oxygen production and Botrytis-induced kinase1 phosphorylation. In addition, complex formation between the MAMP receptor flagellin sensing2 and its signaling partner brassinosteroid insensitive1-associated kinase1 is observed in flg22-treated lecrk-VI.2-1 mutants. LecRK-VI.2 is also required for full BABA-induced resistance and priming of PTI. Our work identifies LecRK-VI.2 as a novel mediator of the Arabidopsis PTI response and provides insight into molecular mechanisms governing priming. |
| Volume | 24(3) |
| Pages | 1256-70 |
| Published | 2012-3-1 |
| DOI | 10.1105/tpc.112.095778 |
| PII | tpc.112.095778 |
| PMID | 22427336 |
| PMC | PMC3336125 |
| MeSH | Aminobutyrates / pharmacology Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis / immunology Arabidopsis Proteins / genetics Arabidopsis Proteins / immunology* Arabidopsis Proteins / metabolism DNA, Bacterial / genetics Disease Resistance Gene Expression Profiling Gene Expression Regulation, Plant Genetic Complementation Test Mutagenesis, Insertional Oligonucleotide Array Sequence Analysis Pectobacterium carotovorum / pathogenicity Plant Diseases / genetics Plant Diseases / immunology Plant Diseases / microbiology Plant Immunity* Plant Stomata / immunology Protein Kinases / metabolism Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / immunology* Protein Serine-Threonine Kinases / metabolism Pseudomonas syringae / pathogenicity RNA, Plant / genetics |
| IF | 9.618 |
| Times Cited | 119 |
| WOS Category | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda01628 |