RRC ID 28120
Author Arase F, Nishitani H, Egusa M, Nishimoto N, Sakurai S, Sakamoto N, Kaminaka H.
Title IAA8 involved in lateral root formation interacts with the TIR1 auxin receptor and ARF transcription factors in Arabidopsis.
Journal PLoS ONE
Abstract The expression of auxin-responsive genes is regulated by the TIR1/AFB auxin receptor-dependent degradation of Aux/IAA transcriptional repressors, which interact with auxin-responsive factors (ARFs). Most of the 29 Aux/IAA genes present in Arabidopsis have not been functionally characterized to date. IAA8 appears to have a distinct function from the other Aux/IAA genes, due to its unique transcriptional response to auxin and the stability of its encoded protein. In this study, we characterized the function of Arabidopsis IAA8 in various developmental processes governed by auxin and in the transcriptional regulation of the auxin response. Transgenic plants expressing estrogen-inducible IAA8 (XVE::IAA8) exhibited significantly fewer lateral roots than the wild type, and an IAA8 loss-of-function mutant exhibited significantly more. Ectopic overexpression of IAA8 resulted in abnormal gravitropism. The strong induction of early auxin-responsive marker genes by auxin treatment was delayed by IAA8 overexpression. GFP-fusion analysis revealed that IAA8 localized not only to the nucleus, but, in contrast to other Aux/IAAs, also to the cytosol. Furthermore, we demonstrated that IAA8 interacts with TIR1, in an auxin-dependent fashion, and with ARF proteins, both in yeast and in planta. Taken together, our results show that IAA8 is involved in lateral root formation, and that this process is regulated through the interaction with the TIR1 auxin receptor and ARF transcription factors in the nucleus.
Volume 7(8)
Pages e43414
Published 2012
DOI 10.1371/journal.pone.0043414
PII PONE-D-12-03604
PMID 22912871
PMC PMC3422273
MeSH Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Cell Nucleus / metabolism Cytosol / metabolism DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism F-Box Proteins / genetics* F-Box Proteins / metabolism Gene Expression Regulation, Developmental / drug effects Gene Expression Regulation, Plant / drug effects Indoleacetic Acids / pharmacology Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Confocal Mutation Plant Growth Regulators / pharmacology Plant Roots / genetics* Plant Roots / growth & development Plant Roots / metabolism Plants, Genetically Modified Protein Binding / drug effects Protoplasts / cytology Protoplasts / metabolism Receptors, Cell Surface / genetics* Receptors, Cell Surface / metabolism Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / genetics* Transcription Factors / metabolism Two-Hybrid System Techniques
IF 2.766
Times Cited 16
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes DNABook