論文 - 詳細
| RRC ID | 28123 |
|---|---|
| 著者 | Takeuchi T, Nemoto-Sasaki Y, Sugiura K, Arata Y, Kasai K. |
| タイトル | Galectin LEC-1 plays a defensive role against damage due to oxidative stress in Caenorhabditis elegans. |
| ジャーナル | J Biochem |
| Abstract |
LEC-1 is a major galectin in Caenorhabditis elegans and contains two carbohydrate recognition domains (CRDs), N-CRD and C-CRD. To determine the role of LEC-1, we examined the phenotypes of a mutant C. elegans strain lacking lec-1. We observed negligible differences in embryogenesis, morphogenesis and egg laying at 20 °C between the mutant and the wild-type. Furthermore, the life spans of the mutant and the wild-type were equivalent at either 20 °C or 25 °C. However, the lec-1 mutant showed a greater susceptibility to H2O2 and paraquat than the wild-type. This result suggests an increased susceptibility to oxidative stress, with the phenotypes being similar to those of lec-10 deletion mutants as previously described. To understand the molecular mechanism underlying this phenotype, C. elegans proteins bound by either the LEC-1 N-CRD or C-CRD were isolated and identified using a nano liquid chromatography-tandem mass spectrometry technique. MIG-6 was identified as a major binding partner of LEC-1 with both N- and C-CRD. From these results and previous reports, we speculate that interaction of LEC-1 and MIG-6 in the pharynx may affect susceptibility to paraquat and that LEC-10 has different functions from LEC-1 in regulating H2O2 and paraquat resistance in the intestine. |
| 巻・号 | 154(5) |
| ページ | 455-64 |
| 公開日 | 2013-11-1 |
| DOI | 10.1093/jb/mvt074 |
| PII | mvt074 |
| PMID | 23935187 |
| MeSH | Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromatography, Liquid Galectins / chemistry Galectins / genetics Galectins / metabolism* Hydrogen Peroxide / chemistry Mutation Nanotechnology Oxidative Stress* Phenotype Tandem Mass Spectrometry |
| IF | 2.476 |
| 引用数 | 6 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1345 tm1262 |