RRC ID 28135
Author Milner MJ, Seamon J, Craft E, Kochian LV.
Title Transport properties of members of the ZIP family in plants and their role in Zn and Mn homeostasis.
Journal J. Exp. Bot.
Abstract A better understanding of the role of the Arabidopsis ZIP family of micronutrient transporters is necessary in order to advance our understanding of plant Zn, Fe, Mn, and Cu homeostasis. In the current study, the 11 Arabidopsis ZIP family members not yet well characterized were first screened for their ability to complement four yeast mutants defective in Zn, Fe, Mn, or Cu uptake. Six of the Arabidopsis ZIP genes complemented a yeast Zn uptake-deficient mutant, one was able partially to complement a yeast Fe uptake-deficient mutant, six ZIP family members complemented an Mn uptake-deficient mutant, and none complemented the Cu uptake-deficient mutant. AtZIP1 and AtZIP2 were then chosen for further study, as the preliminary yeast and in planta analysis suggested they both may be root Zn and Mn transporters. In yeast, AtZIP1 and AtZIP2 both complemented the Zn and Mn uptake mutants, suggesting that they both may transport Zn and/or Mn. Expression of both genes is localized to the root stele, and AtZIP1 expression was also found in the leaf vasculature. It was also found that AtZIP1 is a vacuolar transporter, while AtZIP2 is localized to the plasma membrane. Functional studies with Arabidopsis AtZIP1 and AtZIP2 T-DNA knockout lines suggest that both transporters play a role in Mn (and possibly Zn) translocation from the root to the shoot. AtZIP1 may play a role in remobilizing Mn from the vacuole to the cytoplasm in root stellar cells, and may contribute to radial movement to the xylem parenchyma. AtZIP2, on the other hand, may mediate Mn (and possibly Zn) uptake into root stellar cells, and thus also may contribute to Mn/Zn movement in the stele to the xylem parenchyma, for subsequent xylem loading and transport to the shoot.
Volume 64(1)
Pages 369-81
Published 2013-1
DOI 10.1093/jxb/ers315
PII ers315
PMID 23264639
PMC PMC3528025
MeSH Adaptation, Physiological / drug effects Adaptation, Physiological / genetics Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / isolation & purification Arabidopsis Proteins / metabolism* DNA, Bacterial / genetics Gene Expression Regulation, Plant / drug effects Gene Knockout Techniques Genetic Complementation Test Homeostasis* / drug effects Homeostasis* / genetics Manganese / metabolism* Manganese / toxicity Mutagenesis, Insertional / drug effects Mutagenesis, Insertional / genetics Mutation / genetics Organ Specificity / drug effects Organ Specificity / genetics Phenotype Plants, Genetically Modified Protein Transport / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Saccharomyces cerevisiae / metabolism Subcellular Fractions / drug effects Subcellular Fractions / metabolism Zinc / metabolism* Zinc / toxicity
IF 5.354
Times Cited 67
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes pda18620 pda15827 pda03164