RRC ID 28160
Author Begum P, Fugetsu B.
Title Induction of cell death by graphene in Arabidopsis thaliana (Columbia ecotype) T87 cell suspensions.
Journal J. Hazard. Mater.
Abstract The toxicity of graphene on suspensions of Arabidopsis thaliana (Columbia ecotype) T87 cells was investigated by examining the morphology, mitochondrial dysfunction, reactive oxygen species generation (ROS), and translocation of graphene as the toxicological endpoints. The cells were grown in Jouanneau and Péaud-Lenoel (JPL) media and exposed to graphene at concentrations 0-80 mg/L. Morphological changes were observed by scanning electron microscope and the adverse effects such as fragmented nuclei, membrane damage, mitochondrial dysfunction was observed with fluorescence microscopy by staining with Hoechst 33342/propidium iodide and succinate dehydrogenase (mitochondrial bioenergetic enzyme). Analysis of intracellular ROS by 2',7'-dichlorofluorescein diacetate demonstrated that graphene induced a 3.3-fold increase in ROS, suggesting that ROS are key mediators in the cell death signaling pathway. Transmission electron microscopy verified the translocation of graphene into cells and an endocytosis-like structure was observed which suggested graphene entering into the cells by endocytosis. In conclusion, our results show that graphene induced cell death in T87 cells through mitochondrial damage mediated by ROS.
Volume 260
Pages 1032-41
Published 2013-9-15
DOI 10.1016/j.jhazmat.2013.06.063
PII S0304-3894(13)00463-9
PMID 23892171
MeSH Apoptosis / drug effects* Arabidopsis / drug effects* Arabidopsis / ultrastructure Benzimidazoles / chemistry Cell Membrane / drug effects Cell Nucleus / drug effects Cells, Cultured Culture Media / chemistry Dose-Response Relationship, Drug Endocytosis Graphite / chemistry Graphite / toxicity* Membrane Potential, Mitochondrial / drug effects Microscopy, Atomic Force Microscopy, Fluorescence Oxidative Stress Propidium / chemistry Reactive Oxygen Species Succinate Dehydrogenase / chemistry Water / chemistry
IF 6.434
Times Cited 18
WOS Category ENGINEERING, CIVIL ENVIRONMENTAL SCIENCES ENGINEERING, ENVIRONMENTAL
Resource
Arabidopsis / Cultured plant cells, genes rpc00008